120 THE ROYAL SOCIETY OF CANADA 
almost unlimited time which is impossible in the case of agar or gelatin 
culture media.” The methods of both seed and soil inoculation were 
followed, the series comprising trials in pots and in the field. 
The soil used in the pot series was an extremely light, sandy loam, 
taken from the field devoted to the experiments and which had not 
carried a legume crop for many years. Its nitrogen content was .101 
per cent. 
Respecting the clover in pots at the date of the first cutting there 
was no appreciable difference in appearance, but as the data show, the 
growth from the inoculated crops was heavier than that from the 
untreated. When cut the second time, there was similarly but little 
difference between the pots. In considering the total weight, the 
yields from the uninoculated and seed inoculated pots are seen to be 
very close—the difference not being sufficient to indicate any material 
advantage from the use of the culture. With the soil inoculated, 
however, the yield was markedly higher than from the untreated pots, 
the increase presumably being due to the use of the culture. 
HILTNER’S CULTURES—RED CLOVER AND ALFALFA 
—POT EXPERIMENT. 


| Clover Alfalfa 

Unin- Seed In- | Soil In- Unin- Seed In- | Soil In- 
| oculated | oculated | oculated | oculated | oculated | oculated 


| 
iGrammes Grammes Grammes Grammes |Grammes | Grammes 
| 




Ist cutting, green. . 97-3 109-7 125-9 58-4 | 55-1 | 939-0 
2nd “ oe Me 73-9 69-3 | 104-4 69-2 | 63-5 71-8 
aay | | | - 
(Totale tee 171-2 179-0 | 230-3 127-6 | 118-6 | 126-8 
The alfalfa pots exhibited no marked differences, at either the first 
or second cuttings and the total yields do not indicate any benefit from 
the use of the culture. 
The pot experiments with pease similarly showed no beneficial 
effect from the culture when cut; the uninoculated and seed inoculated 
crops were very much alike in appearance and were decidedly superior 
to the soil inoculated crop, a deduction confirmed by the yields. The 
data are as follows: Uninoculated 279.2 grms.; seed inoculated 264.8 
grms.; soil inoculated 221.3 grms. 
