[MA VOR] PROTOZOAN PARASITES 67 
showed two kinds of granules; large colorless granules up to 8u in 
diameter and smaller greenish granules 1 to 1-5u in diameter. The 
greenish granules are by far the most numerous. When stained with 
Giemsa’s azur-eosin the first are a deep red, the second yellowish in 
color. No haematoidin or other crystals were found,!an inclusion 
described in M. lieberkühni in European pike by Cohn (96). Also 
the ‘“Mesoplasma”’ described by the same author as between the ecto- 
plasm and endoplasm was not observed. The absence of these two 
characters may be due to the particular period during which the para- 
site was studied by the writer. The nuclei which measure from 1 to 
2u in diameter are surrounded by a deeply staining membrane and 
contain three or four chromatin granules staining deeply with haema- 

Fig. 2. Myxidium lieberkühni, Butschli from the urinary bladder of the pike, 
Lucius lucius. a-e, myxosporidia free in the urine; f, spores; g-h, myxosporidia 
attached to the epithelial wall of the bladder; i, myxosporidium detached from wall; 
a-f, drawn from fresh preparations; g-i, drawn from sections of the urinary bladder 
stained with Delafield’s haematoxylin. a-e, x 660. f, x 1420. g-i, x 1480. 

1, The author has since found haematoidin crystals in myxidium lieberkühni 
from pike caught in Wisconsin. 



