68 THE ROYAL SOCIETY OFSCANADA 
toxylin. The nuclei vary greatly in number, the number seeming 
not to correspond in any way with the size of the myxosporidium. 
Some of these nuclei undoubtedly show division, but the details of 
this process have not been worked out. No pansporoblasts, sporo- 
blasts, or spores were found in the myxosporidia. 
The spores, only two of which were found floating freely in the 
urine, are spindle shape with the axis of the spindle shape with the axis 
of the spindle (in this case at right angles to the axis of the spore as 
defined by Thelohan, ’95) curved (Fig. 2,f). The measurements of 
the spore are, width (the long axis of the spindle) 17-18u length 
(measured at the middle at right angles to the axis of the spindle) 5x. 
The polar capsules, of which there are two, one at either side of the 
spore, measure 4 in length. They each contain four or five coils 
of filament. No attempt was made to extrude the filaments. The 
spordplasm fills the rest of the spore. In one case two nuclei could 
be clearly seen in the sporoplasm (Fig. 2, f.). 
The characters given above enable the parasite to be recognized 
as Myxidium lieberkühni, Butschli found in Lucius luctus in European 
waters. For descriptions of the M. lieberkühni see Bütschli (82) 
and Cohn (96). 
C. Myxosporidian from the urinary bladder of Stizostedion 
vitreum. 
This myxosporidian was found in the urinary bladders of each of 
three pike-perch (Stizostedion vitreum, Mitchill), being the total num- 
ber in which the urinary bladder was examined microscopically. 
Like the parasite of the pike it seems to be nonpathogenic. The para- 
site was found both free in the urine, (Fig. 3, a-d) and attached to the 
wall of the bladder (Fig. 4). The free forms vary greatly in shape, 
being rounded, elongated or branched. The largest measured 2001 
in its greatest diameter. An ectoplasmic layer was clearly to be dis- 
tinguished. In certain cases this layer was produced into fine pro- 
cesses, such as might serve for attachment (Fig. 3, d). In a stained 
smear the parasite was found attached to the epithelial cells, by a large 
area of ectoplasm which stained rather deeply (Fig. 4). In the fresh 
state the endoplasm was filled with greenish granules. Stained 
preparations show very numerous nuclei measuring 1-5 to 2u in dia- 
meter in the larger myxosporidia. In many respects the myxospori- 
dium resembles that of Myxidium lieberkiihni found in the pike. 
No spores or sporoblasts were identified. Without knowing the 
method of spore-formation and the structure of the spore it is impos- 
sible to assign the parasite to its genus and species. So far as the writer 
