56 ROYAL SOCIETY OF CANADA 
but as a rule it formed only two or three colonies. In the winter, 
fungus mycelia often developed without any accompanying bacteria. 
Up to the present we have not paid any special attention to the 
species of the micro-organisms. However, the bacteria flora of Winnipeg 
is extremely varied. This is shown by the differences in colour and 
modes of growth of the bacterial colonies. Both pink and white yeasts 
are frequent. Among the fungi we made out the following species: 
Cephalothecium roseum, Stysanus stemonites, Macrosporium  clados- 
porioides, Mucor racemosus, M. mucedo, M. stolonifer, Pencillium glaucum 
and Aspergillus glaucus. There were several other species which, on 
account of sterility, we were unable to identify. One of these, which 
was not infrequent, produced a colourless mycelium without spores, 
but had the peculiarity of turning the gelatine a blood-red colour. 
EXPERIMENTS AT St. CHARLES. 
As has already been stated, St. Charles is a small village six miles 
west of the centre of the city of Winnipeg. The exact place where 
the experiments were made was a piece of bare prairie at a considerable 
distance from any human habitations. A single experiment by the 
volumetric pump method was made monthly during the summer and 
at intervals of six weeks during the winter. The following is a tabulated 
statement of the results. For the sake of comparison, the results of 
experiments made in Winnipeg on some of the same days have been 
added. 


Number in 10 litres | Number in 10 litres 
Month. at St. Charles at Winnipeg. 
DING TR OEM LEO A 15 40 
Meili: are cite eee es ene 8 18 
AI PUSt Art coisa Gere oes 14 62 
Sentem bors eae eee wea ee ee 9 30 
CODES Dex nie URL RSR eg Ne AN NE 12 
November SOUSSE UE Er ER 3 
Mamet ber ay tr TAME diront 0 
HABEHAUY stots foe our eee 0 me 
PIS ae ESS I EEE 2 3 
Mana Mein Euros TE 4 24 

It thus appears that there are about three times as many organisms 
in the air of the City of Winnipeg as there are out on the prairie six 
miles away. This can be readily understood when one thinks of the 
