32 THE ROYAL SOCIETY OF CANADA 
Methods for the examination of udder tissue. 
Immediately after slaughter, the whole gland was removed, wrapped 
in a clean towel and taken to the laboratory. Here the outside was 
thoroughly wiped with a disinfectant. Small pieces were then cut from 
the interior with sterile instruments and placed in Petri dishes. The 
agar and litmus were added, the whole agitated for a short time, and 
allowed to solidify. Incubation in all cases was at blood heat, and the 
plates were examined on the third day. 
Bacteria isolated from the udders of heifers. 
During the course of this investigation we had the opportunity 
of examining the bacterial content of the udders of heifers, age from 1 
to 2 years, which had never calved. For brevity’s sake these are termed 
virgin udders, and their bacterial content was as follows:— 
Udder No. 1.— à 
M. lactis albidus (Conn.) 88% of the total number of bacteria present. 
B. ramosus liquefaciens. 
Bact. proteum. 
Bact. striatum.? 
Udder No. 2.— 
Galactococcus versicolor var. B. 
M. varians lactis. 
M. lactis albidus. 
Udder No. 3.— 
M. lactis acidi. 
M. lactis citreus. 
M. varians lactis. 
Udder No. 4.— 
A small unidentified bacillus that digested milk and stained at the 
poles only. 
Udder No. 5.— 
M. lactis albidus. 
M. varians lactis. 
M. rosaceus—in very small numbers. 
The bacterial content of the udders of seven cows. 
The following notes are made from careful observations on several 
animals in the College herd, during a period of about three months, 
under summer conditions. The animals were dealt with singly. 
