[HARRISON-SAVAGE] BACTERIAL CONTENT OF THE UDDER 33 
COW—“ Lizzie.” 
A perfectly normal grade Ayrshire cow, udder healthy, teats fairly 
Strippings. 
10 milkings. 
Only one sterile sample was obtained. 
M. lactis albidus found in the milk of all quarters at other times. 
Middlemilk. 
4 milkings. 
Quarters 1, 2 and 3 gave pure cultures of J. lactis albidus. 
Quarter 4 yielded only M. varians lactis. 
Foremilk. 
From all the quarters together the following were isolated.— 
B. mesentericus fuscus (see Conn). 
B. subtilis. 
B. acidi lactici. 
M. albidus. 
Unfortunately, at this stage the animal developed trouble with her 
udder, which, though not caused by pyogenic organisms was of an in- 
flammatory nature; hence no further notes were taken. 
The animal was killed thirteen weeks later, and the udder examined. 
The resulting plates showed M. lactis albidus and M. varians to be pres- 
ent in different portions of the gland. Cultures made from tissue close 
to the teat and cistern developed colonies of a bacillus in addition to the 
micrococci mentioned, though such colonies were not numerous. This 
organism was not identified, but was probably one of the subtilis group. 
It was a sporulating bacillus, formed long chains, and digested milk. 
COW —«Spotty.” 
An easy milking grade Ayrshire cow giving about 24 lbs. daily 
during observation. Udder well developed and perfectly normal. 
Strippings. 
8 milkings. 
M. lactis albidus found in varying numbers on each occasion in the 
milk of quarters 1 and 2. M. varians lactis found only once in 
either of these quarters. 
Quarters 3 and 4 apparently sterile. 
Sec. IV., 1911. 3. 
