36 THE ROYAL SOCIETY OF CANADA 
Four and one half months later the white and brown cocci were 
isolated from the left front quarter. The latter organism had assumed 
a lighter tint and was closer to a dun colour than to chocolate. Upon 
cultivation at 40 degrees C. for a few days, it became yellow and re- 
sembled M. varians closely, but did not digest milk. Plates from the 
rest of the udder were sterile, except from the lower portions, which 
contained a spore-bearing rod. 
COW .—“Lady Edith.” 
A young cow, in perfectly healthy condition, udder normal but 
not particularly well developed. 
Strippings. 
Quantities of 20 c.c. taken at 11 consecutive milkings showed no 
growth even after 12 hours incubation at 37 degrees C. 
Middlemilk. 
4 milkings. 
M. lactis acidi in milk of quarters 1 and 2. 
M. lactis aureus in milk of quarters 1 and 2. 
Foremilk. 
The teats were carefully sealed over with surgical plaster after a 
thorough sponging with strong corrosive sublimate, and the milk as a 
result of this treatment showed no change of flora as long as the plaster- 
ing was continued. Eight milkings were thus observed. 
When milked as usual, however, the following results were found :— 
B. mesentericus fuscus (see Conn.) 
B. subtilis. 
M. lactis rugosis. 
The milk of all quarters being taken together, only two milkings 
analyzed. 
The animal was not killed. 
COW .—“Linburne Fizzy.” 
Fairly hard milking Ayrshire, giving about 25 lbs. daily. Udder 
well developed, but not in any way exceptional. 
Strippings. 
4 observations. 
M. lactis albidus in very small quantities (5-10 per c.c.). 
Middlemilk. 
As above. 
