164 ROYAL SOCIETY OF CANADA 



The Growth of B. typhosus and other Oryauisms in ^Esculin Media. 



We were able to obtain a number of varieties of B. typhosus, 

 Paratyphosas, B. dysenferiae^ etc., from Dr. J. G. Adami, Professor 

 of Pathology, McGill University, and Dr. C. W. Duval, formerly 

 pathologist of the Montreal General Hospital, and we wish to thank 

 these gentlemen for their interest in our work, and to record our 

 appreciation of their kindness. 



The cultures sent us were as follows: 

 1064 B. typhosus isolated from gall bladder, autopsy 131 — 1908. 



308 " " " bile at operation. 



965 " " " gall bladder, autopsy 108—1908. 



9652 " " " gall bladder 



496 " " " mesenteric gland. 



1011 " " " gall bladder, autopsy 119—1908. 



407 " " " blood ante-mortem. 



370 " " " a case of osteomyelitis. 



330 " " " bile. 



1011 " " " liver substance, autopsy 119 — 1908. 



896 " " " by Dr. Duval. 



1064 " " " from gall bladder, autopsy 131—1908. 



345 B. paratyphosus isohited from an appendix abscess. 



526 " " " urine — case of cystitis. 



997 " " " a pus cavity in abdomen, autopsy 116 



—1908. 



973 " " " a subcutaneous abscess, autopsy 109 



—1908. 



488 " " " an abscess from hip. 



— B. paratyphosus isolated by Longicope, Philadelphia. 



— " B " 



— " Paracolon (?) Source unknown. 



B. dysenteriae (Duval) a lactose fermenting and mannite sphtting 

 variety (C). 



1 " (Flexner) isolated in the Philippines — a mannite 



splitter. 



B. fecalis alkaligenes isolated by Duval. 



B. of Hog Cholera— (Th. Smith). 



B. of Asiatic Cholera from A. C. Abbott, Philadelphia, 



All of these species and varieties were first grown on sets of 

 ordinary media to see if they were typical in their biological charac- 

 ters. For this purpose the following media constituted a set, — Beef 

 peptone gelatine, beef peptone agar, glucose neutral red broth, htmus 

 lactose broth, saccharose broth, litmus milk, litmus whey, Dunham's 

 solution (for indol). 



