[HARRISON & vanderleck] ^SCULIN bile salt MEDIA 16S 



It seems unnecessary to record any data concerning this test, 

 except the deviations from normal characters. All the varieties of 

 typhoid were normal; para typhoid — 345 was undistinguishable from 

 typhoid; paratyphoid 526 gave slight traces of indol; paratyphoid 

 997 gave a strong indol reaction, paratyphoid 973 and 488 gave a 

 little gas and fluorescence in neutral red glucose broth, acid reaction 

 in litmus lactose, and indol reaction; paratyphoid a gave a little gas 

 and fluorescence in neutral red glucose, and an alkaline reaction in 

 milk in 12 days; paratyphoid /?. gave a little gas and slight fluores- 

 cence in neutral red glucose broth, strong alkaline reaction in milk 

 (12 days), and an indol reaction. 



The tests of these organisms were made on several combinations 

 of the œsculin media — both solid and liquid, and also includes the 

 simple synthetic media for the isolation of B. coli from water suggested 

 by Dolt (4), and modified by the addition of sesculin. Fuller par- 

 ticulars follow. 



A. J^sculin-agar, made up according to the formula already 

 given. 



B. ^Esculin-jjeptoney made up of water, 1% peptone, 0.25% 

 iron citrate, 0.1% sesculin — acidity -\- 1.0. 



C. ^Esculin-peptone-bile-salt, made up of water, 1% peptone, 

 0.5% commercial bile salt, 0.25% iron citrate, 0.1% aescuUn — 

 acidity -\- \.0. 



D. jEsGulin-j)eptone-Liehig, made up of 1% peptone, 0.2% 

 Liebig's Meat Extract, 0.25% iron citrate, 0.1% sescuHn, — acidity -\- 

 1.0. 



E. jEaculin-Liebùj-hile-salt, made up of 1% peptone, 0.2% 

 Liebig's meat extract, 0.5% commercial bile salt, 0.25% iron citrate 

 and 0.1% sesculin — acidity -)- 1.0. 



F. uEsculin-glycerine, etc., made up of agar 1.5%, glycerine 

 0.5%, ammonium phosphate 0.1%, iron citrate 0.25%, sescuHn 0.1% 

 — acidity + 0.4. 



G. jEsculiii-ammoniuvi - lactate, etc., made up of agar 1.5%, 

 ammonium lactate 0.5%, disodium phosphate 0.1%, iron citrate .25% 

 and sesculin 0.1% — acidity -\- 0.4. 



The last two media F. and G. were made according to the 

 directions of Dolt (4), but with iron citrate and sesculin added. 

 In the table, B. coli isolated from sewage, fseces and river-water 

 are included for the sake of comparison. 



These results show that the typhoid bacillus, as well as paraty- 

 phoid forms, do not produce black colonies on sesculin media. These 

 organisms grew well, however, presenting characters similar to those 



Sec. IV., I'.IO'J. 10. 



