[HARRISON A VANDEHLECK] 



.ESCULIN BILE SALT MEDIA 

 Growth of B. Typhosus axd Other Organism in ^sculin Media. 



Organism 



B. coli from sewage. 



106 4 B 



30S B. 



river water . 



t}-phosxis 



typho 



965 B 



965- B. 



496 B, 

 1011 B, 



407 B. 



370 B, 



330 B. 



typhosus . 

 typhosus . . 

 . typhosus . 

 ■. typhosus. 



. typhosus. 

 . typhosus. 

 . typhosus. 



1011 B. typhosus.. . 



896 B. typhosus,. . 

 1064 B. typhosus.. . 

 345 Paratyphosus 

 526 Paratyphosus. 

 997 Paratj-phosus . 

 973 Paratyphosus. 

 488 Paratyphosus . 



Paratj-phosus a. 



Paratj-phosus ,3 



-Esculin Agar Plates 



Paratyphosus (Paracolon). 



B dysenteric Du val 



B .dvsenteriœ (Flexner',.. . 

 B. fecalis alkaligenes . 



B. of Hog Cholera 



Black colonies with field 



Good growth, no black co- 

 lonies in 72 hours. . , 



B of Asiatic Cholera. . 



^Esculin Peptone 



Jet black in 24 hours . 



No blackening in 72 hrs . 



-I^culin Peptone Bile Salt 



Jet black in 24 hours. , 



No blackening in 72 ho 



White colonies, good 

 growth 



No change in medium . . 



No change in medium . 



D 



-.'Esculin Peptone Liebig 



Jet blank in 24 hours. . 



Turbid precipitate, no blackening in 72 hours . 



No blackening in 72 hours. 



No blackening in 72 hours, plight brown colour 12 days 

 Turbid precipitate; no blackening in 72 hours 



yEsculin Liebig Bile Salt 



Jet black in 24 hours., 



Turbid precipitate, but no 

 blackening in 72 hours.. 



vEsculin glycerine 



^Esculin am. lactate 



Jet black colonies, small, . Black colonies, small. 



No black colonies Brown colonies, 72 hours , 



Jet black colonies j Black colonies. 



Very shght growth, no | Very slight growth, no 

 black colonies in 72 hrs.; black colonies in 72 hrs. 



No visible growth in 72 h 



No blackening in 72 hrs.. 



Turbid precipitate, no 

 blackening m 72 hrs. 



No blackening in 72 hrs., clear light brown in 12 days No blackening 72 hrs. clear, 



' light brown 12 days . 



No blackening in 72 hours. 



No blackening in 72 hn 



Very heavy dense ppt. 24 hrs. ppt. settled, occupy J^ Very hea\'y dense ppt. 24 



amount in tube 72 hrs. no blackening ; lirs. settled in 72 nrs. no 



blackening 



No change in medium . 



No change in medium. . . 



Good growth, no black 

 colonies 



Slight growth, no black 

 colonies 



Slight growth, no black co- 

 lonies in 72 hours. . . . 



No visible colonies in 't 

 hours 



No visible growth, 72 hrs. 



Very slight growth, no 

 black colonies. 



No visible colonies in 72 hrs. 

 No visible colonies in 72 hrs. 



Good ^owth, no black 

 colonies. 



Slight growth, no black 

 colomes . 



