270 THE ROYAL SOCIETY OF CANADA 



This conclusion has been checked by examining the filtrate from 

 maximal cultures of yeast in media containing various proportions 

 of wort; from solutions containing ten per cent, or less, the filtrate 

 contains no bios; there is a little bios left in the filtrate from twenty 

 per cent, solution, in this case reproduction has been checked by the 

 alcohol formed; similarly with higher percentages up to pure wort. 

 The amount of bios in one litre of wort is thus shewn to be sufficient 

 for the rapid growth of about 280 grams of yeast. 



These results have been used to standardize the method proposed 

 by Mr. Clark for the quantitative determination of bios, and the 

 method has been used to check the losses which occur during the 

 production of concentrated preparations of bios. 



The Reaction of Acenaphthene with Phthalic Anhydride and Aluminium 



Chloride 



By F. Lo^RiMAN, B.A. 



Presented by Professor F. B. Allan, F.R.S.C. 



Naphthalic anhydride with benzene and aluminium chloride 

 gave naphthalic acid only, and 4-bromnaphthalic anhydride with 

 benzene and aluminium chloride gave only 4-bromnaphthalic acid. 



Phthalic anhydride with aluminium chloride and acenaphthene 

 in benzene solution gave a good yield of an acid whose silver salt 

 contains 33.1% silver. When 5-bromacenaphthene is used instead 

 of acenaphthene an acid is obtained which v/ill be further investi- 

 gated. 



Some Derivatives of Maleic and Fumaric A cids 



By H. Oddy, M.A. 



Presented by Professor F. B. Allan, F.R.S.C. 



Rubidge and Qua's method for the preparation of aromatic 

 lactones gave negative results when maleic anhydride, benzene and 

 aluminium chloride were used. Several new derivatives of acrylic 

 acid have been prepared by using maleic anhydride, aluminium 

 chloride and naphthalene, anthracene, and diphenyl, respectively. 



