36 THE ROYAL SOCIETY OF CANADA 



I 



was no great difficulty in training the animal to lie quietly on his 

 side during the observation and great care was taken to see that 

 there were no leaks around the edges of the mask. 



The usual procedure was to determine the quotient several 

 times, then to administer cane sugar or pure dextrose either by 

 mouth or subcutaneously, with or without injections of insulin and 

 then to observe the quotient at frequent intervals. Observations 

 have so far been made on five animals, at periods varying from 48 

 hours to 154 hours after the pancreatectomy. We are aware that 

 several observers have found that the depancreated animal still 

 retains, for 4-5 days, some power to utilize carbohydrate as shown 

 by a small rise in the quotient when sugar is ingested. This, however, 

 does not detract at all from the value of our results. 



The following are the most significant results: 



Dog //.—Before pancreatectomy, R.Q., after 22-23 hours starva- 

 tion was 0.85 and 0.86. 30 gms. sucrose by mouth caused it to rise 

 to 1.0 in 35 minutes and it remained exactly at this level for 2 hours. 

 The animal was then depancreated (Jan. 21st) and the R.Q., 48 hours 

 later (Jan. 23rd), was 0.63 rising to 0.76-0.78 in 1| hours after 

 20 gms, sucrose. In 74 hours (10.46 a.m.) after pancreatectomy 

 25 gms. sucrose (by mouth) and 10 c.c. insulin, subcutaneously, 

 caused the quotient to rise to 0.86 in 31 minutes and 0.90 in 1| hours; 

 it then fell to 0.77 in 3-3| hours. The animal was again given 

 20 gms. sucrose and 8 c.c. insulin at 3.15 p.m. and the R.Q. rose within 

 50 minutes to 0.91 then to 0.94 (1 hr. 37 min.), 0.93 (3 hrs. 17 min.) 

 and 0.87 (4 hrs. 1 min.). Next morning (25th) R.Q. stood at 0.68 

 and 20 gms. sucrose raised it to 0.82 (3 observations) and 0.85 (1 

 observation). On the 26th, R.Q. was 0.68-0.72; 20 gms. sucrose 

 raised it to 0.81 in 1 hour, and 10 c.c. insulin 5 hours later caused it 

 to rise to 0.90 in 40 minutes. 



The earlier results of this experiment are not entirely convincing 

 because there was a definite increase in R.Q. with sucrose alone. 

 This may be because sufficient time had not elapsed since the pan- 

 createctomy for the power to utilize carbohydrates (especially 

 laevulose) to disappear. The observations on Jan. 26th, five days 

 after the pancreatectomy are more satisfactory. 



Dog III. — R.Q. 0.65 (49 hrs. after pancreatectomy) ; after 7 c.c. 

 insulin subcutaneously (without sucrose) it rose to 0.70 (1 hr. 34 min.), 

 0.68 (1 hr. 51 min.) and 0.67 (2 hr. 7 min.). 20 gms. sucrose, given 

 orally, 5 hours after the insulin caused the quotient to rise to 0.89-0.86. 



A repetition of this experiment on the next day raised R.Q. 

 to 1.06. 



