40 THE ROYAL SOCIETY OF CANADA 



being 0.85. In 16 depancreated dogs the average was 0.7; in one 

 case it was 1.05 per cent. Macleod and Prendergast found in two 

 normal dogs that the glycogen in the ventricle is increased by starva- 

 tion to 1.00 and 1.05 per cent, respectively. In the present investiga- 

 tion, 0.79-0.92 per cent, and 0.98 per cent, glycogen were found 

 respectively in the hearts of two depancreated dogs fed sugar 

 but receiving no insulin. In four other depancreated animals to 

 whom insulin was given, as well as sugar, the values were 0.725, 

 0.600, 0.570 and 0.296. These few observations indicate that insulin 

 reduces the glycogen percentage in the heart of diabetic animals 

 to within the normal limits. 



With regard to the skeletal muscles, nothing conclusive can as 

 yet be said although there is some indication that insulin causes the 

 percentage of glycogen to increase (cf. table). 



II. Total Fatty Acid in Liver, Heart and Blood 



Fat. — This has been determined as fatty acid by Leathes modi- 

 fication of the Kumagawa-Sato method with the following results, 

 the animals in all cases being given large amounts of sugar. 



Although much larger percentages of fat than these have been 

 observed to occur in the liver after phosphorus or phloridzin it is 

 nevertheless much above the average for laboratory animals, which 

 is given by Leathes as about 4-6 per cent. With regard to the blood 

 our results compare with those given by Bloor for severe diabetes 

 viz., 1.01 per cent. These values were decidedly altered in animals, 

 receiving insulin thus: 



There were two dogs, however, in which the liver fat was not 

 found to be reduced following insulin. In both of these (53 and 54) 

 excessive doses of insulin were given so that the one animal (53) died 

 during the night and the other (54) in the forenoon following the 

 administration. In the former case 10.276 per cent, and in the latter 

 26.360 per cent, of fatty acid were found in the liver. 



