. 126 THE ROYAL SOCIETY OF CANADA 



available, in the form of shredded codfish, or fillets. A certain amount 

 of salt was present in these products, which amount was disregarded 

 when adding different percentages of salt by weight, but which added 

 about two per cent, salt to the finished media. One part of fish was 

 added to two parts distilled water, allowed to digest over night, 

 autoclaved, and then filtered through a cloth. Such filtration gave 

 better results than when a paper filter was used, the amount of fish 

 remaining in the broth encouraging growth. 



To the codfish filtrate salt, agar, small percentages of peptone, etc., 

 were added. With high concentrations of salt the amount of agar 

 was increased, and at times considerable difticulty was experienced 

 in getting the agar into solution. Gelatine could not be used at all 

 on account of the action of the salt v/hich prevented solidification. 

 All media were sterilized in the autoclay at 15 pounds pressure for 

 20-30 minutes. 



On codfish agar containing 16 per cent, to 35 per cent, salt growth 

 at 37° C. was slow. In 7 days the amount was moderate, filiform, 

 slightly raised, glistening, smooth, translucent, bright red in colour, 

 odour somewhat unpleasant, consistency viscid, the medium un- 

 changed. Growth at 22° C. was less in amount, but otherwise 

 similar. No growth in agar containing less than 15 per cent. salt. 



On 16 per cent, salt peptone agar, without codfish, the growth 

 was very scant after 7 days incubation at 37° C., with the same 

 characteristics as above, but with less colour. 



There was no growth on potato slants tubed in brine of various 

 percentages- — -5, 10, 15, 20, 25, 30 and 35 per cent. 



Hoye found that Torula epizoa, and also a red organism he had 

 isolated, developed well on a special medium made from 30 parts 

 flour, 35 parts salt, and as little water as possible to make a stiff 

 paste. This medium was tried, but, despite massive inoculations, the 

 attempts to culture our red organism were unsuccessful. 



Shredded salt cod was used in making two other media: The 

 first one, made from one part fish to three parts cooked potato, and 

 10 per cent, solar salt with enough milk added to moisten and hold 

 together this paste; the second made from 66 grams of egg white, 

 100 grams of fish and 10 per cent, solar salt. These two were tubed 

 and sloped, sterilized and then inoculated, but no growth developed 

 on either medium. 



Pieces of dried salt cod were cut and tubed in 16 per cent, salt 

 solution in a manner similar to the preparation of potato tubes, 

 sterilized, and then inoculated. After incubation for seven days at 

 37°C. the growth was red, moderate, spreading, slightly raised, 



