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THE ROYAL SOCIETY OF CANADA 



The red pigment was soluble in solar salt solution, absolute 

 methyl alcohol, ethyl alcohol, acetone, and slightly soluble in water; 

 insoluble in sodium chloride (C.P.) ether, xylol, chloroform, or weak 

 acetoic acid. 



Relation to Oxygen. — The organism is an obligate aerobe. It 

 will not grow in the closed arm of the fermentation tube, or under the 

 surface of agar. We have never seen submerged colonies in agar. 

 A cover glass lowered on the brushed surface of an agar plate is 

 sufficient to prevent growth under it. On salt codfish broths with 

 strips of filter paper, the colour is produced well above the surface 

 of the liquid. On infected fish the organism is always on the surface, 

 and colour is never seen on fish kept well under the brine in puncheons. 



Relation to Nitrogen. — 16 per cent, solar salt solution was sterilized 

 in tubes. In each tube a strip of filter paper was placed half in and 

 half out of the brine. To these tubes were added various sterilized 

 solutions, so that each tube contained 0.1 per cent, or 0.5 per cent, 

 of the specific solution. All tubes were then heavily inoculated 

 in quadruplicate and incubated at 37° C, for 25 days, with the 

 following results : 



16% solar salt — control No growth. 



16% salt (C.P. metal free) — control . No growth. 



16% solar salt +0. 1 per cent, potassium nitrate No growth. 



" ' .No growth. 



,. No growth. 



No growth. 



" ammonium chloride No growth. 



" No growth. 



" " tartrate No growth. 



" No growth. 



" asparagin , .No growth. 



No growth. 



" peptone (Difco) Growth. 



" Growth. 



There was more growth on the 0.1 per cent, peptone than on the 

 0.5 per cent, peptone. 



This series shows that this organism is a peptone bacterium. 



Hydrogen Ion Concentration. — A series of 16 per cent, salt fish 

 broth tubes were prepared with varying pH; eight tubes of the same 

 pH. In each tube was placed a piece of tested filter paper, acid free, 

 half in and half out of the liquid. All were inoculated and incubated 

 at 37° C. for 21 days with the following results: 



