[HARRISON & KENNEDY] DISCOLOURATION OF CODFISH Ï35 



agar incubated at 37° C. for two days growth was moderate, filiform, 

 and rather pale yellow colour; and, at 22° C. for the same time,, it 

 was practically the same. Colonies on agar plates were punctiform. 

 No gas was produced in lactose, maltose, glucose or saccharose. Fish 

 agar containing varying percentages of solar salt were inoculated and 

 incubated at 37° C. for two days with the following results: on agar 

 containing no salt, 5 per cent, and 10 per cent, salt, growth was 

 abundant; on 15 per cent, it was less, but still fairly abundant; 

 on 20 per cent, it was only moderate; on 25 per cent, and on 30 per 

 cent, it was slight; and on 35 per cent, very slight. There was also 

 a gradual change in colour, from a bright vivid yellow on agar to 

 which no salt had been added, citrinus on 5 per cent, and 10 per cent., 

 sulphureus on 15 per cent, and 20 per cent., to a cream shade on 

 25 per cent., 30 per cent, and 35 per cent. In many respects this 

 organism resembles Micrococcus luteus of Winslow and Winslow, 

 except that in colour ours was more sulphureus than luteus (Saccardo). 



When plating out from reddened salt cod many yellow colonies 

 developed on 16 per cent, salt fish agar plates, in three days, at 37° C. 

 They were round in form, about 1.5 mm. in diameter, when full 

 grown, with smooth surface, raised elevation, and entire edge. A 

 microscopical preparation, stained with gentian violet, showed the 

 presence of forms which resembled torulae, rather oval shaped, 

 frequently appearing in twos, side by side. It averaged 2 ;u in dia- 

 meter. 



Luteus (Saccardo) coloured colonies also developed on fish agar 

 plates containing 16 per cent, solar salt; and microscopical prepara- 

 tions from such colonies, stained with methylene blue, showed the 

 presence of cocci, slightly more than 1 )U in diameter, which were 

 Gram positive. Growth on beef peptone agar slopes, incubated at 

 37° C. for two days, was abundant, slightly spreading, raised and 

 glistening, with a definite orange colour. Incubated at 22° C. for 

 two days growth was practically the same. From a gelatine stab 

 inoculation there was infundibuliform liquefaction. In both milk 

 and litmus milk, incubated at 37° C. for two days, there was an 

 orange ring around the top, and a deposit of the same in the bottom, 

 but otherwise there was no change in these media, even at the end of 

 seven days. In beef broth incubated at 37° C. for two days, there 

 was a ring around the top, and considerable clouding through the 

 medium. On fish agar containing 16 per cent, solar salt, and also 

 on brine agar, both incubated at 37° C. for two days, growth was 

 abundant, slightly spreading, raised and glistening. On the former 

 the growth was luteus colour (Saccardo) (20) while on the latter it 



