136 THE ROYAL SOCIETY OF CANADA 



was more ochraceus (Saccardo). On agar plates colonies were 

 punctiform, mostly under the surface. No gas was produced in 

 glucose, lactose, maltose or saccharose. Fish agar slopes, containing 

 varying percentages of solar salt, were inoculated, and incubated at 

 37° C. for two days, with the following results: On agar containing 

 no salt, 5 per cent, and 10 per cent., growth was abundant, filiform 

 and luteus colour ; on 15 per cent, there was less growth, but still fairly 

 abundant and luteus in colour; on 20 per cent, growth was slight, 

 ochraceus colour; on 25 per cent., 30 per cent, and 35 per cent, growth 

 was very scant, but approached ochraceus in colour. With the 

 exception of colour production the cultural characteristics of this 

 organism approach those of Micrococcus citre'us of Winslow and Win- 

 slow (31). 



On some of the fish agar plates Isabellinus colonies developed, 

 which, when transferred, appeared very pale in colour, at first, but 

 in older cultures gradually assumed the darker colour, Isabellinus, 

 which had first attracted attention. Microscopically, this organism 

 was a medium-sized rod, but further knowledge of it is yet to be 

 ascertained. It was found also in Turks Islands brine. 



Hoye's Sarcinomyces Islandicus was isolated from some samples 

 of fish. 



A fîesh-coloured colony frequently was observed on the salt fish 

 agar plates, which, when examined microscopically, showed the 

 presence of cocci. They stained well with methylene blue, and also 

 were Gram positive. On beef peptone agar sloped cultures, incubated 

 at 37° C. for two days, growth was moderate, filiform, flesh colour. 

 From gelatine stab inoculations growth was filiform, but very scanty 

 with no liquefaction. In beef broth there was slight clouding, flaky 

 precipitate, and no growth at the top. No changes developed in 

 either milk or litmus milk. On a sloped culture of fish agar containing 

 16 per cent, solar salt, growth was filiform, more abundant than on 

 beef peptone agar, and flesh colour. On brine agar growth resembled 

 that on fish agar, and was again more abundant than on beef peptone 

 agar. There was no gas produced in glucose, lactose, maltose or 

 saccharose. On fish agar containing no salt, growth was beadlike, 

 more appearing near the top of the slope. On 5 per cent, and 10 per 

 cent., growth was abundant; on 15 per cent, it was slightly less than 

 10 per cent.; on 20 per cent, still less; on 25 per cent., 30 per cent., 

 35 per cent, there was still growth, but very scant. 



These were the most numerous forms occurring, but a number of 

 other organisms appeared from time to time on our plates. Evidently 

 there is a large number of halophilic organisms, and some of them 



