152 THE ROYAL SOCIETY OF CANADA 



EXPLANATIONS OF PLATES 



PLATE I 

 Fig. 1. — -Cured codfish, showing marked red discolouration (the dark areas). 

 Fig. 2. — Photograph of petri dish containing 16 per cent, salt codfish agar, 

 showing colonies of the red organism and salt crystals (reduced.) 



PLATE II 



o 



o 



Fig. 1. — Ps. salinaria. From 25 per cent, salt codfish agar, 7 days at 37 

 Fig. 2.— Ps. salinaria. From 16 per cent, salt codfish agar, 7 days at 37 

 (Antigonish). 



PLATE III 



Drawings of Pseudomonas salinaria, magnified approximately 1000 times. 

 1. — From a culture in 16 per cent, salt codfish agar, 9 days at 37° (Annapolis). 

 2. — From a culture in 16 per cent, salt codfish agar, 2 days at 37° (Annapolis). 

 3.— From a culture in 16 per cemt. salt codfish agar, 2 days at 37° (Annapolis). 

 4. — From a culture in 25 per cent, salt codfish agar, 9 days at 37° (Annapolis). 

 5. — From a culture in 35 per cent, salt codfish agar 9 days at 37° (Annapolis). 

 6. — From a culture in 25 per cent, salt codfish agar, 3 days at 37° (Annapolis). 

 The third transfer in 25 per cent, codfish agar. 



PLATE IV 



Drawings of Pseudomonas salinaria. 



1-8. — Round cells of various types. All preparations stained with Giemsa. 



1. Dark violet centre, blue margin. 



2. Dark violet centre, blue margin. 



3. Violet with very dark ring. 



4. Ring beginning to break up into granules. 



5. Ring completely fragmented into granules. 



6. Protoplasm and granules being discharged. 



7. Another type with smaller granules, staining pink. 



8. An earlier phase of 4. 



9. — Above, showing transition from cylindrical to round type. Below, another type. 

 10. — Types of cells showing diffuse nucleus. 



(c) Fibrillar protoplasm with chromatic granules at intersections. 



(b) Axial filament of chromatin, with granules around the periphery. 



(c) Cell b breaking up. 



11. — Amorphous symplasm, at times faintly stained or deeply stained (violet), 

 giving appearance of a contoured colony. 



12. — Symplasm with granules, some staining more deeply than others, granules 

 round, often in pairs or threes and small rods. 



13. Large cylindrical cells forming from symplasm. 



14. Large cells forming a membrane, which shows as a dark broken line at 

 first, later becoming continuous. 



15. Protoplasm stains with Giemsa pale red at first; later stains darker red 

 violet, and is granular. 



16. Rods staining faintly pink, with dark violet granules of various sizes. 



17. — Cell membrane disappears, and a granular mass results; granules violet 

 on a pink-violet mass. 



All preparations stained with Giemsa. 



