60 THE VENOM OF HELODERMA. 



Venom, Dialyzing 49 hours. 

 Outside Dialyzer. 

 Rat 109, 105 g. 



June 26, 5^ 10'° iEJected subcutaneously 1 c.c. of solution. 6 o'clock slightly 

 affected. June 27 recovered. 

 Mouse 28. 



June 26, S^ 11'^ injected subcutaneously 0.2 c.c. of solution. G o'clock affected, 

 but much less than mouse 29. June 27 recovered. 

 Inside Dialyzer. 

 Rat 110, 110 g. 



June 26, 6^ 12" injected subcutaneously 1 c.c. of solution. 6 o'clock lying down, 

 very sick. June 27 dead. 

 Mouse 29. 



June 26, ^^ 13'° injected subcutaneously 0.2 c.c. of solution. 6 o'clock nearly 

 dead. June 27 dead. 

 Venom Dialyzing 67 hours. 

 Rat 112, 100 g. 

 June 27, 11'' 30" injected subcutaneou.sly 1 c.c. of solution. 13 o'clock lying 

 down. 13*' 30"' lying down. 1 o'clock lying down. 2 o'clock improving. 

 June 2S recovered. 

 Mouse 30. 



June 27, ll' 30° injected subcutaneously 0.2 c.c. of solution. 12 o'clock lying 

 down. / o'clock better. June 22 recovered. 

 Rat 113, 110 g. 



June 27, 11'' 32'" injected subcutaneously 1 c.c. of solution. 12 o'clock lying 

 down; about same as rat 112. 12'' 30"' lying down, not much worse than rat 

 112. 1 o'clock worse than rat 112. 2 o'clock dead. 

 Mouse 31. 



June 27, 11'' 33'" injected subcutaneously 0.2 c.c. of solution. 13 o'clock lying 

 down. 1 o'clock dead. 

 Venom Dialyzing 112 hours. 

 Rat 84, 85 g. 



June -, 10'' 52'" injected subcutaneously 2 c.c. of solution. 2 o'clock sick, but 

 may recover. June 14 well. 

 Rat 88, 120 g. 

 June -, 11''20"' injected subcutaneously 1 c.c. of solution. 1^20'" seriously 

 affected. June 14 recovered. 

 Rat 85, 85 g. 



June -, 10'' off^injected subcutaneously 2 c.c. of solution. 2 o'clock dead. 

 Rat 89, 130 g. 



June -, //'' 22'" injected subcutaneously 1 c.c. of solution. 1'' 20'° dead. 

 Mouse 13. 

 June -, 12^44™ injected subcutaneously 0.2 c.c. of solution. 1'' 50'" seriously 

 affected. 2'' 35"' dead. 

 Mouse 14. 

 June -, 12'' 43'" injected subcutaneously 0.2 c.c. of solution. 2'' 20"' dead. 



EFFECT OF FILTRATION. 



The venom of Heloderma passes unchanged through a Chamberland filter. 

 Two similar portion.s of venom, one filtered, the other unfiltered, produce, when 

 injected into animals, identical results, that is to say, they bring about .similar 

 sj'mptoms and kill in approximately equal times. We found in ten rats which 

 we injected that exactly similar results were produced by equal doses of filtered 

 and unfiltered venom. 



Again, it may be noted that the venom of Heloderma acts in a manner 

 similar to the venoms of the Colubridse, which pass through a Chamberland 

 filter, while that of the Viperitlse does not. 



Filtered Venom. 



Rat 31, 140 g. 



June 5, 4*' 24'" injected intraperitoneally 0.1 c.c. venom. 6^30'" drowsy. 

 June 6, a. m., found dead. 



