64 THE VENOM OF HELODEEMA. 



Rat U, 9S g. 



May 31, 12^ 10'° injected subcutaneously 0.1 e.c. venom in 1 c.c. NaCl 0.S.5 per cent. 



12^ 30™ lying on side; breathing rapid. 13^ 40"' lying on side; breathing slow. 



ygb ^^ni j-eflexes present. /'■OO™ reflexes absent. ^i" 70™ better; able to move 



about. 2^ 00"' lies on side; apparently dying; reflexes pre.sent. 2^ 30"' dead. 



Dog 1, 10 kg. 



May 31, 3^ IJ,'" given subcutaneously 0.1 c.c. fresh venom. 3^ 25"' vomited; urine 

 and feces voided. 3'" SO™ lying down; very sick. S*" 50" vomited. June 

 1, died during forenoon. 

 Bog 2, 9700 g. 



May 31, 3^ 17'" given subcutaneously 0.02 c.c. fresh venom. 3'' 30"' lying down; 

 trembles; occasionally much twitching. 3^ 40"' voids urine and feces. 

 Recovered. 

 DogS, 11 kg. 



May 31, ,3^30"' given 1.1. c.c. fresh venom subcutaneously. 3^ 4^"' vomited; urine 

 and feces voided. Very sick all afternoon. Died diu'ing night. 

 Cat 1, 3000 g. 



JuneI, ^i*" 70" give subcutaneously 0.4 c.c. fresh venom. 7^'';?0" vomits. 12'"2.5"' 

 pupils enormou.sly dilated; vomits. 7.2'' 30" very nervous; cries if touched; 

 breathing difficult. 2^ 00"' breathing rapid, superficial, pupils still dilated. 

 Sick for some days, but recovered. 

 Cat 2, 3500 g. 



June 1, 12^ OS"' given subcutaneously 0.09 c.c. of fresh venom. 12^ 25"' pupils enor- 

 mously dilated. 7.2'' 40™ breathing difficult. ^iiOO" breathing rapid, super- 

 ficial, pupils dilated. Recovered. 

 Cat 3, 3000 g. 



Jc^fE 1, 12^ 06"' given .subcutaneously 0.04.5 c.c. of fresh venom. 12'' 25"' pupils enor- 

 mously dilated, vomits. 12'' 40'" i)upils contracting. Recovered. June 

 24 sick, killed; no lesions. 

 Cat 4, 3000 g. 



June 3, 2'' 12"' given 0.9 c.c. of fresh venom subcutaneously. 2^ 20"' violent move- 

 ments of head from side to side, cries, pupils enormously dilated, eyes roll. 

 2^35'" more quiet; eyes still roll, but head movements stopped. 2'' 5-5"' lies 

 quiet, very nervous; makes crying movements, but has nearly lost voice; breath- 

 ing difficult, audible. 3'' OS'" no longer able to hold up head; voice gone. 

 June 4 living; takes no food. June 6 dead. 

 Cat 7, 1500 g. 



June 8, 12^55"' injected intraperitoneally 0.6 c.c. of fresh venom. 7''0.j"' ]5upils 

 greatly dilated, losing voice. 7'' 40'" pupils contracted, voice gone; breathing 

 labored; tears secreted. Recovered. 

 Cat .9, 700 fir. 



June 8, 2'^ 25"' injected intr.aperitoueally 0.7 c.c. of fresh venom. 2'' 35"' very much 

 affected; pupils dilated; tears and saliva flowing; breathing forced. 5'' 00"' 

 has remained in apparently dying condition all afternoon; breathing forced; 

 nearly paralyzed. 5^ 30"' dead. 



CONDITION OF MOUSE'S EYE PRODUCED BY INJECTION OF 

 HELODERMA VENOM. 



AVe found that the injection of heloderma venom into mice caused fre- 

 quently a pecuHar condition of the eye. This condition did not appear as a 

 rule when mice were injected with a large cjuantity of venom — a quantity 

 which was letlial within a comparatively short time — but appeared usually 

 when quantities of venom had been injected which caused death after many 

 hours or several days. At times the injection of a sublethal dose of venom was 

 sufficient to produce this lesion. However, its appearance was not constant, 

 even after the injection of a lethal dose. 



The lesion was observed as early as from I5 to 6 hours after the injection 

 of the venom. At this time the ej'eball appeared somewhat more prominent 

 than usual and the cornea showed a slight opacity. The protrusion of the eye 

 and opacity of the cornea progressed fairly rapidly (there was, however, con- 



