198 THE VENOM OF HELODEBMA. 



trolling this were similar tubes with increasing and decreasing doses of com- 

 plement. 



(1) 0.5 c.c. serum and 0.5 c.c. venom and .15 c.c. complement. 



(2) 0.5 c.c. serum and 0.5 c.c. venom and .12 c.c. complement. 



(3) 0.5 c.c. serum and 0.5 c.c. venom and .10 c.c. complement. 



(4) 0.5 c.c. serum and 0.5 c.c. venom and .08 c.c. complement. 



(5) 0.5 c.c. serum and 0.5 c.c. venom and .06 c.c. complement. 



Incubate for 2 hours at 37° C, then add 1 c.c. washed erythrocytes and 2 units of 

 amboceptor and incubate again for 1 hour at 37° C. 



Result: Result: 



(1) Complete hemolysis. (4) No hemolysis. 



(2) Complete hemolysis. (5) No hemolysis. 



(3) Partial hemolysis. 



CONCLUSIONS. 



In all four tests made and using both systems, no true fixation of comple- 

 ment could be determined. In no case was more complement absorbed than 

 the sum of that fixed by the venom and serum alone. By this method, there- 

 fore, the presence of an antibody can not be demonstrated. 



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