338 Annals Entomological Society of America [Vol. VII, 
therefore less liable to live, if it be placed at any other tempera- 
ture, longer than if it has been living constantly at this secondary 
temperature. 
The rapid starvation at the high temperature has morpho- 
logically rejuvenated the insect but has rendered it physiologi- 
ally old. This slowing down and probably also change in 
function has rejuvenated and removed the cytoplasmic ob- 
stacles while morphological age, due to destruction of reserve 
products, has gone on to a less extent than at the high tempera- 
ture. At medium temperatures, there is no change in rate nor ~ 
a great enough degree of starvation to remove these inactive 
substances—therefore the cell is not rejuvenated morphologic- 
ally and is physiologically old. In other words, the insect is 
older than the insects prepared by either of the other two 
methods. 
PART 3: 
EFFECTS OF EXPOSURE TO TWO DIFFERENT TEMPERATURES ON 
HIBERNATING INSECTS. 
In part 2 of. this article, certain studies of the effects of 
temperature upon the longevity of starving insects were made. 
In this part, I propose to further substantiate the statements 
made by the results obtained from certain experiments on the 
hibernating brood of the Codling moth larve (Carpocapsa 
pomonella L.) 
The experiment was started with larve collected from wind- 
fall apples gathered under the trees and sent by the courtesy 
of Mr. Frank Perry of Sebastapol, Sonoma County, California, 
where the insects were collected. These insects were taken 
in the late part of July, 1913, and many of them pupated. Be- 
lieving these to be of the earliest second brood, the experiment 
was abandoned and begun over again with larve that were 
collected in the cocoon—all the two hundred and fifty larve 
of the second experiment were collected in one mass of cocoons 
under a packing house. There could be no doubt then as to 
their hibernating condition and as to the similarity of their 
exposure to temperature, humidity and disease. 
The larve were handled in the following manner: the co- 
coons were opened and two larve dropped into each clean test 
tube which was then plugged with cotton. The test tubes 
