264 Report S.A.A. Advan'cement of Science. 



the post-mortem examination was made, there was in addition some 

 emphysema of the apices and free edges of the Imigs. 



The pericardium contained an excessive amount of yellow serous 

 fluid. 



No abnormal condition was seen in the abdominal ca\ity except 

 the spleens which were slightly enlarged, the liver which was in 

 both cases congested and friable, and some exudation of serous 

 material into the omentum and mesentery. 



No micro-organisms of any kind were found in the blood. 

 This occurrence was somewhat surprising to us both and I there- 

 upon determined upon attempting once more the infection of clean 

 cattle with Horse-sickness from the horse. 



Accordingly I took several animals of a new consignment to the 

 Institute and then under rareful conditions carried out the experi- 

 ments. 



On the 4th February 1898, Mr. Robertson and 1 inoculated a 

 clean young ox with 30 c.c. of fresh Horse-sickness blood which we 

 injected into the jugular vein. Some reaction occurred during the 

 first few days after which the temperature fell, but on the i6th day 

 it rose. The maxima were as follows till the moment of death. 



J 6th day 106' 4 



i7th .. io6'6 



i8th loy'o 



19th .. 107*2 



20th 106' 2 



-ist died. 



"The post-mortem was of iiiierest inasmuch as nearly ever)' 

 s\mptom of Horse-sickness was rejjroduced, the interlobular pul- 

 monary effusi(jn. the pleural and pericardial effusions. " (Vide Report 

 of the Director of the Colonial Bacteriological Institute for the Year 

 1898.) 



Ten c.c. oi the Ijlood of this ox wa.s used to inoculate 1)\ sub- 

 cutaneous injection, horse No. 122. After an incubation j)eriod of 

 eight days, the temperature rose, and the animal died, on the 13th 

 day, of typical Horse-sickness. 



'Ten c.c. of the l)lood of this ox was al.so u.sed, bv intravenous 

 injection, t(j inoculate a second ox, in which the temperature rose 

 -to 106.4 ("1 the Jith day, to 107.4 un the 12th. and which we killed 

 when dying of the disease on the 16th day. 



At that time, having succeeded so completelv in transferring 

 the disease to cattle, I tried also to infect goat.s. 



The goats, however, were bt»rn in this area, and are more 

 insusceptible than goats taken from other areas. 



Of the several goats inoculated none died but most had severe 

 reactions. 



One of these goats, which had been ino<nilate(l with jo r.v.. sub- 

 cutaneously, of ]>reserved horse-sickness l)lood, and developed a high 

 temperature as a result, was bled on the roth dav after inoculation. 



A \oung ox was inoculated with 30 c.c of "this blood l)v intra- 

 venous inoculation on the i8th Febniarv. 1808. 



