28o Report S.A.A. Advancement of Science. 



on the eighth day and parasites were found in the blood on the tenth 

 day. 



Horse 2g. This horse was inoculated also from Horse B which 

 however had not been re-inoculated so that its blood was drawn 51 

 days after inoculation. The temperature began to rise on the ninth 

 day and parasites were found on the tenth day. 



Horse 30. This animal was inoculated with the blood of Horse 

 27 on the 14th of January. The blood of Horse 27 at this period 

 shewed very few parasites. The temperature began to rise on the 

 ninth day and parasites were found in the blood on the tenth day. 



Horse ji. This horse was inoculated with 20 c.c. of the blood 

 of " salted " horse A (which had not been re-inoculated since the 

 previous experiments, about a year previously and which since that 

 date had been kept in an isolation stable carefully groomed and 

 tended). The temperature began to rise on the tenth day and 

 parasites were found on the eleventh day. 



I now decided to re-inoculate this horse which was accordingly 

 done with 10 c.c. of preserved blood and ten days later it was again 

 bled and this horse (31) re-inoculated with 20 c.c. of the blood. On 

 the tenth day following a slight rise of temperature occurred which 

 immediately subsided. 



I desire to lay considerable stress on this experiment because 

 it has been suggested that the fever which follows the use of the 

 blood of a " salted " horse that has been recently re-inoculated is due 

 to the Horse-sickness, while the parasites are of the nature of 

 accidental infection. In the present case we find fever induced by 

 the blood of a salted horse which has not been inoculated for a 

 period of about a year, while the blood of the same " salted " horse 

 after re-inoculation fails to produce any degree of fever in the horse 

 formerly inoculated with its blood. 



Horse 32. I now took preserved virulent blood and inoculated 

 a cl*>an goat with 10 c.c. by intravenous injection. Ten days later 

 this goat was bled and immediately the blood was mixed with equal 

 portions of sterile glycerine and water. Twenty-four hours later I 

 injected horse 32 with 10 c.c. of this blood. On the ninth day a rise 

 of temperature began to be apparent and on the tenth day I found 

 perfect specimens of the parasite in the blood. 



Horse jj. This horse was inoculated with 20 c.c. of the blood 

 of salted horse B which had not been re-inoculated since the former 

 occasion eighty-two days previously. The temperature began to rise 

 on the ninth day and parasites were found on the eleventh day. 



Horse J4. I now took a horse which had been inoculated in 

 the former experiments (a year ago) on repeated occasions with salted 

 blood and also with blood in which parasites had been found. That 

 horse however never shewed any parasites. I now bled this horse 

 and inoculated horse 34 with 20 c.c. of its blood. On the ninth, 

 tenth and eleventh days slight rises of temperature occurred and on 

 the 1 6th day I found a few but typical parasites in the blood. The 

 reaction in this case was but slight and the parasites very few as 

 compared with the horses which had been inoculated from " salted " 



