B22 
and Trees’? (1910). It may be significant, in view of what 
follows, that Massee’s set of figures of the Macrosporiwm includes 
a drawing of three Fusarium spores found, according to the text, 
‘mixed with, but no proof of relationship with, the Macro- 
sporium.’’? As far as can be judged from the figure, these 
Fusarium spores resemble those subsequently referred to in this 
paper, and also those figured by Dr. Van der Bijl in his account of 
a South African wilt of carnations.* The Uganda wilt under dis- 
cuission is undoubtedly similar in all respects to that described by 
Van der Bijl, and it is unnecessary, in view of the previous publi- 
cation of his paper, to go into full details. 
ISOLATION OF THE FUNGUS. 
In attempts to show whether the Uganda wilt was the same as 
that of South Africa or to discover another cause for it, if such 
existed, small pieces of tissue were cut with sterile instruments 
from the underground diseased areas of wilted carnations, already 
described, and, after sterilisation in mercuric chloride (1: 1000) 
or flaming in alcohol, were shaken up in tubes of solid media and 
poured into petri-dishes. The solid media consisted of 2} per 
cent. carnation-, prune-, or sugar-cane-agar. 
Mycelial growth took place at laboratory temperature after the 
lapse of twelve hours, and microconidial formation was very 
vigorous after three days. After fourteen days the conidia were 
three-, and, later, multi-septate and of a Fusarium type. 
Chlamydospores were produced in abundance both terminally and 
intercalarly. The aerial mycelium, at first faintly pink in the 
mass, became after twelve days of a deep pink colour. 
Fic. 1.—Mycelium, conidia and conidiophores of 
sp 
- < c § Of Fusarium 
- from Carnation, in pure culture, 56 hours, x 
500. 
Subcultures were subsequently made on banana plugs and 
r slants, and the latter were used in inoculation experi- 
ments. The colour of the banana-plug mycelium was at first 
3 Yee digs Bid i. Wilt” or “Oeowa Roo’ Dank 2 we | 
Fisarium ep-—Autals AGEL Bicone, Te Ser tne Re ened bY 
