323 
faintly grey, and afterwards pink, while that of the prune-agar 
mycelium was a very faint yellow succeeded by a pink. lamy- 
dospores appeared in these cultures in six days. The fungus was 
also grown from microconidia placed in sterile distilled water. In 
this case the mycelium assumed a rosy-pink colour which became 
almost dark-red. It was many-septate, globuled and apparently 
brittle, and numerous conidia and chlamydospores were 
developed. 
At the same time whole diseased carnation plants were care- 
fully sterilised and afterwards placed in damp chambers; these 
were found to develop upon and in the neighbourhood of the 
sunken area of the crown aerial mycelium of a white-pink colour 
accompanied by conidia after the lapse of twelve days. This 
mycelium and the conidia were indistinguishable from those 
obtained from the cultures mentioned above, and from those found 
in further cultures derived from them by transferring mycelium 
and conidia directly into tubes and plates. 
‘ISOLATION OF THE FUNGUS FROM WILTED NIGELLA AND DELPHINIUM. 
_, A few days after the receipt of the wilted carnation material, 
dying plants of love-in-a-mist(Vigella)and larkspur (Delphinium) 
_were found in the same bed of the same garden which afforded the 
examples of carnation wilt, and, after a further few days, a dying 
plant of Cosmos (bipinnatus?) was similarly collected. 
All these were found to show the same macro-symptoms as the 
wilted carnations, and, histologically, to be attacked and per- 
meated in the same parts by mycelium similar to that found in 
the carnation material: On the exterior of two of the Delphinium 
tap-roots were found mycelium, conidia, and chlamydospores 
which were shown to be identical with material of cultures 
derived from the Delphinium plants: No mycelium or sclerotia 
of Sclerotium Rolfsii were found on either the Nigella, Delphi- 
nium or Cosmos material, 
Whole plants of Nigella and Delphinium were sterilised by 
immersion for ten minutes in mercuric chloride solution (1: 1000), 
and small pieces of tissue from the blackened areas near the crowns 
were cut out under sterile conditions and placed in prune-agar 
plates. The remains of the plants were put into damp-chambers. 
Th all of these, both Nigella and Delphinium plants, plentiful 
mycelial growth occurred on collars and tap-roots after the lapse 
of thirty-six hours, and after a further twelve hours Fusarium 
: Rowe om er. : not | 0 re A ere pee an yy = 
essive crops of them did not attain, the size, of those used to 
oculate the banana plugs until fier twenty-four days. The 
a2 
