THE FROG Td 
very young frog or toad, recently metamorphosed. Fix in 
Muller’s or Tellyesnicky’s fluids. Decalcify with 70 per cent. 
alcohol containing 10 per cent. nitric acid. Stain in bulk with 
borax carmine or with paracarmine. In cutting the sections be 
careful to orient the object so as to secure exact cross-sections, 
some of which will pass longitudinally through the columella. 
Cut rather thick sections (about 15»). 
16. Cross-sections of entire frogs. Obtain some very small 
frogs or toads recently metamorphosed, fix in Tellyesnicky’s fluid, 
decalcify, stain in toto with borax carmine or with paracarmine 
and cut the entire body into thick transverse sections (15 to 
20pm). As a review exercise have students sketch sections taken 
at random, and label the parts. 
