APPENDIX II 



477 



blue, etc. Take a watch glass and pour in some of the 

 solution, which is best diluted, immersing the sections, and 

 keep the sections immersed a fair time ; then wash with 

 water or alcohol, according as water or alcohol dissolves the 

 stain. More than one stain may be employed successively, 

 using the necessary solvent for each stain to wash out any 

 excess. 



For permanent mounting, glycerine, glycerine jelly, Canada 

 balsam, and dammar may be used. In the case of glycerine 

 the slide may be sealed or merely transferred to glycerine 

 jelly. Dilute glycerine consists of half glycerine, half water 

 Or take for glycerine 2 parts glycerine, i part glacial acetic 

 acid, boiled together. The cover is fixed to the slide by a 

 coat of gold size, Brunswick black, Canada balsam, dissolved 

 in benzol or xylol, applied to the edge with a brush. The 

 section is placed in glycerine and kept under a bell jar till 

 the water has evaporated. Similar methods are adopted with 

 the other media. Each slide must be carefully numbered 

 and labelled. 



The following is a list of necessary apparatus : 



Fine-pointed scissors. 



Forceps. 



Razors. 



Scalpels. 



Section-hfter. 



Mounted needles. 



Camel-hair brushes. 



Watch glasses. 



Reagent jars. 



Test-tubes, beakers. 



Spirit lamp. 



Black and white mounting 



tile (enamelled). 

 Glass slides (3 in. x i in.). 



Cover slips (| in.). 



Blotting-paper. 



Drawing - paper (or Bristol 



board). 

 Hard pencils (HHH). 

 Labels for slides. 

 Slide box or rack. 

 Bell glass. 

 Lens. 



Compound microscope. 

 Micrometer. 

 Objectives (i in., i in., | in., 



iin.). 

 Camera lucida. 



