56 THE ENTOMOLOGIST. 
as dissection proved, the little larve, as in many cases of 
attempts to breed from hybrid parents, were unable to break the 
ege-shell. In fact, in my own numerous experiments, I have 
only known two cases where progeny resulted from such a cross. 
Next, what about the winnt 3 X subsignaria 2 pairing? The 
case is parallel with that of the primary pairing quercinaria 3 
X subsignaria 2. The defect is mechanical, and the attempted 
pairing results in fatal internal injury to the female. Our third 
possible pairing gives us a combination of both of the defects of the 
other two cases. ‘The female is seriously injured, but still is not 
prevented from laying eggs, some of which are fertile but unable 
to yield their little larve. From all of these considerations one 
can conclude that, sooner or later, with multitudinous experi- 
ments one would secure progeny by means of this hybrid male. 
(c) The Segregation in the Secondary Forms.—It is clear that 
here, whilst we are concerned with some scheme of inheritance 
based on the plan worked out by Mendel—so ably expanded by 
Bateson, Punnett, and others—we are not dealing with a pair of 
single allelomorphic characters. We have, in the formation 
of the F; gametes, a total segregation of almost all the quercinaria 
characters (with the possible exception of the colour factor) 
from those of subsignaria. To put it plainly, we have a linking 
of almost the whole block of quercinaria characters, and a similar 
linking of all the subsignaria characters; in other words, we 
can talk of ‘‘ quercinarianess’’ and ‘‘ subsignarianess.” The 
gsametes of the pure species may be represented as § and Q 
respectively, and consequently the zygotes of the F, generation 
as SQ, which, in the absence of dominancy of § or Q, are to a very 
great extent midway between the parents—an observation exactly 
paralle! to the well-known case of the blue Andalusian fowl, and 
such as has usually been found to occur when one crosses two 
distinct species. That this was not incompatible with the correct 
segregation of the gametes in the F) generation was proved by 
Bateson and Punnett’s experiments with Andalusian fowls, and, 
what is more important, it is indicated, but not discussed, by 
Denso* in his account of the secondary hybrid between Celerio 
hyb. galiphorbie and Celerio euphorbie. In the F, generation 
Celerio hyb. galiphorbie was intermediate, whilst in that of F, 
segregation occurred—a, case exactly the same as the present. 
To proceed, the gametes of the pure species are all Q and 
all S respectively, whilst half of. those of the hybrid will be 
Q and half S. Hence, in crossing quercinaria g with 
winnt 2, the gametes of quercinaria will meet in fertilisation 
exactly one-half of their number of Q and a similar number 
of §.. There ought, therefore, to be produced, if fertilisation is 
successful, an equal number of zygotes of composition QQ and 
SQ, and similarly, when subsignaria male is used, we should have. 
* Bull. Soc. Lep. de Genéve, vol. i, fase. 4, p. 308. 
