134 MEMOIRS OF THE NEW YORK BOTANICAL GARDEN 



Wager ('04), Gregory ('05), Nichols ('08), Darling ('09), and 

 others that the nucleole by budding forms chromosomes or 

 chromosome material. Fine fibers appear in close proximity to 

 the nucleus. I have not, however, found a definite felted zone. 

 In some sections there is only the faintest indication of fibers 

 present as shown in Fig. 32a, b, c. I have not traced the pro- 

 gressive development of the spindle. The spindles in the first 

 division are larger but otherwise are like those of the somatic 

 divisions. The chromosomes form a very evenly arranged equa- 

 torial plate stage. FiG. 11 represents a polar view of a spindle 

 and ten chromosomes can be counted. The chromosomes are 

 quite angular and a definite longitudinal cleft divides the tetrads 

 into dyads (Figs. 10, 20, 33). At this time no abnormalities 

 appear either in the cytoplasm or on the spindles. There is no 

 indication of extranuclear nucleoles or portions of displaced 

 chromosomes. Fig. 12 represents the dyads approaching their 

 respective poles. Each chromosome appears longitudinally split, 

 undoubtedly in preparation for the second division. After the 

 chromosomes have reached the poles the central spindle persists 

 in many cases and show all the peculiarities described by Went 

 ('87) and Timberlake ('00). It is not uncommon, however, to 

 find radial fibers coming from the chromosome groups (Fig. 23) 

 and forming an irregular aster. Chromosome-like bodies now 

 make their appearance in the cytoplasm and on the spindle fibers. 

 I have observed them in Drosera rotundifolia, D. intermedia, and 

 D. filiformis and only during the division phases. In color, size, 

 and shape they remind one, however, of the belated chromosomes 

 of Strasburger ('82), Juel ('00), or the unpaired chromosomes of 

 the Drosera hybrid of Rosenberg ('04, '09) and the Oenothera 

 hybrid of Geerts ('09). They bear perhaps an even greater 

 resemblance to the nucleole and look like the figures of extra- 

 nuclear nucleoles of Zimmerman ('93) and more recently Allen 

 ('05) for Lilium. The size of these bodies varies; while some 

 of them are about the diameter of the chromosomes others are 

 smaller. They are spherical in shape but have a tendency to 

 become angular. By their staining reaction it is difficult to 

 determine whether they resemble more the chromosomes or the 

 nucleole. By a careful study of a great number of these stages 

 I am convinced that these bodies take a nucleolar stain. The 



