366 MEMOIRS OF THE NEW YORK BOTANICAL GARDEN 



In 1913, the plants grown in the previous year were retained 

 and se\-eral sets of seedlings grown from the crossed seed. These 

 plants were tested as fully as conditions permitted for self-sterility 

 and for fertility among themselves, and careful records kept of all 

 manipulations. The evidence that self-sterility was the rule in 

 these plants may be presented in table i. Here the plant is named 

 and data given regarding the number of heads pollinated, the 

 number of dates upon which pollinations were made, and the 

 number of heads with no seed or with seed. 



The results were very conclusive for these plants. The number 

 of individual heads tested is high, ranging from 10 to 40 for a plant. 

 The plants A, B, C, Ej, and E22 were also tested in 1914 (as well 

 as in i9i2,of which no record was kept), and, with the exception 

 of one head of plant C, not a single seed was produced. 



Similar data could be given for other plants. None of the 29 

 plants of the cultivated common chicory (E series) nor of the 27 

 plants of the A series (progeny of open-fed seed of plant A) proved 

 to be self-fertile in any degree. Twenty-five other plants, progeny 

 of plants B and C, tested for self-fertility gave the same results. 

 It will be noted that further instances of self-sterility appeared in 

 the Fi, Fo, and F3 generations. Furthermore, self-sterility ap- 

 peared as the rule in all the varieties of chicory grown at the 

 breeding plots. In 1913, about twenty-five plants were grown of 

 each of the following varieties: (i) Magdeburg, large-rooted; 

 (2) Magdeburg, strap-leaved; (3) Witloof; (4) improved spotted; 

 (5) improved red-leaved Treviso; (6) improved striped-leaved; 

 (7) long cylindrical-formed giant; (8) improved large-leaved; (9) 

 improved white; (10) large-rooted Silesian, and (10) common or 

 wild chicory (Barbe de Capucin). The seeds of these varieties 

 were furnished by the J. M. Thorburn Company, and was obtained 

 by them from France and Germany through their foreign agents. 

 Self-pollinations were tried on several plants (usually five) of each 

 of these varieties, but in no case was a plant found to be self- 

 fertile in any degree. Crosses between many of these plants 

 failed quite as is shown in table i. 



Careful examination was made of the mature pollen of many 

 plants, including all the plants mentioned in table I. Very 

 few shrivelled grains were found and it was very evident that 

 impotence in respect to pollen production is not the cause of 



