258 A - B - Stout Cytoiogia 3 



and 14 are reported. Haploid numbers of more than 12 are frequent in 

 various genera with, in various cases, rather conclusive evidence of poly- 

 ploidy. Thus in Ornithogalum, species with 6, 8, 9, 14, 16 and 27 haploid 

 chromosomes are reported, and also there are variations in numbers 

 for individuals of certain of the species. The somatic numbers in 

 Hyacinthus, especially in the cultivated clons, are reported to include 

 such numbers as 18, 19, 21, 22, 23, 24, 25-26, 27, 28, 30 and 36. Here, 

 however, the so-called basic set of 8 is itself composed (Darlington, 1926) 

 of 4 classes in respect to size and position of constrictions in the num- 

 bers 3 : 1 ; 2 : 2, which is evidence of irregular and incomplete duplica- 

 tion. The loss of one chromosome of the haploid set characteristic of a 

 genus seems evident in the two species of Allium which have a number 

 of 7. Marked variation within a species seems evident in various cases 

 such as in Tricyrtis hirta for which numbers of 6, 12-13 and 13 are all 

 reported. It is obvious that in the genera of Liliaceae the numbers of 

 chromosomes suggest the basic numbers of 4 and 6 from which many 

 grades of polyploidy have developed. 



For the near relatives of Hemerocallis, haploid numbers of 12, 16 

 and 24 have been reported for species of Hosta (including Funkia). 

 Also the haploid number of 12 is the rule for all the species of Lilium 

 except that the clon called Lilium tigrinum is reported to be a triploid 

 of 36 somatic chromosomes (Takenaka and Nagamatsu, 1930). The 

 haploid number of 12 in various genera could have developed either by 

 a duplication of a basic number of 6 or by a triplication of the still 

 lower number of 4, and in either case a loss of one could give the com- 

 pliment of 11 seen in Hemerocallis. 



The chromosomes in Hemerocallis are very similar in size and shape 

 and they offer few or no distinguishing features that allow ready 

 identification of possible duplication or fragmentation as is the case for 

 certain other genera of the Lily Family, as Hyacinthus (Darlington, 

 1926) and Yucca (Bonnet, 1911). In the processes of sporogenesis in 

 the triploid Europa Daylily there are extreme variations in the number 

 of chromatin masses. In the early stages of the equatorial plate of the 

 first of the reduction divisions as low as six aggregates are to be 

 observed. In the late stages of the second division groups of 5, 6 and 

 7 chromosomes are frequent. It is definitely known that spores with 

 the low number of 6 chromosomes may survive at least to the stage of 

 the division of the primary nucleus. There is thus numerous and 

 various groupings of chromosomes in numbers below as well as above 

 the number, 11, all of which strongly suggests that some lower number 



