22 



daily observations as to the number of young, but I have the follow- 

 ing notes on the development of the colony : — 



August 3, I count 15 cocoons and see a number of eggs and 

 larvse; Aug. 8, I count 21 cocoons; Aug. 17, three callows have 

 emerged ; Aug. 20, there are 5 callows today. 



August 25. There are at least 10 callow workers today. They 

 are very active and have excavated a tunnel nearly 6 inches in length 

 around the bottom of the glass jar. They have moved some of the 

 brood about 1/2 mches from the original cell of the queen. 



August 28. There are 15 callow workers. Very active. Their 

 main tunnel is about 10 inches in length and is started upward. It 

 is half-way to the top of the cylinder. 



August 29. They have excavated to the top of the sand. 



I did not break up this nest in order to get the exact count, but 

 the approximate count at the end of the season was 15 to 17 workers 

 and I larva. No pupjie or eggs. 



The seven cases in which the queen succeeded in founding a 

 colony and living through the season are as follows. 



This gives an average of 11 workers produced by a queen in one 

 season, with a maximum of 27. The average number of eggs laid 

 by the queens in the four cases in. which I was able to get the en- 

 tire count, is 193.5. The first-year workers are very small, on account 

 of insufficient nourishment. 



The above data show that sexual forms are not produced the 

 first year. It is not at all likely that they are produced the second 

 year because of the very greatly increased amount of nourishment 

 required for producing them. After the second year the average and 

 maximum colonies probably increase very rapidly, as the number of 

 workers is then large enough to provide plenty of nourishment for 

 the queen to lay a much larger number of eggs. 



