271 
tissues in the form of streaks was always present, but it was 
difficult, owing to the general brown colour of diseased parts, 
to trace the streaks from one part of the plant to another. Only 
chlamydospores were seen within the tissues. 
. palmarum, Cke., was present upon them. On only one or 
two seeds, mycelium accompanied by microconidia which 
recalled strongly those of the carnation Fusarium was found. 
It seemed possible that the germination of those seeds had been 
prevented by attacks of the Fusariwm in the soil of the beds, 
but whether this was definitely so could not be determined. The 
few seeds obtained were so permeated by moulds that sterilisation 
had to be thoroughly done, so thoroughly that, in every case, 
whatever living organisms may have been within were killed. 
ISOLATION OF THE CASHEW Nut FUSARIUM AND ITs 
GROWTH IN PURE CULTURE. 
Fresh material when surface-sterilised by careful washings 
in successive portions of rain water, distilled water, and mercuric 
chloride solution (1: 1000), and placed in a moist atmosphere, 
showed plentiful typical mycelium in tufts of a honey-yellow, 
white, or pale-pink colour after two or more days. The mycelium 
frequently took the form of coremial strands. Those plants of 
which the hypocotyls were in a collapsed, soft, and watery 
condition gave the best results. The first mycelial tuft invariably 
appeared at the base of the hypocotyl, and it bore a few micro- 
conidia after about five days (Fig. 1). 
Fig. 1. Typical microconidia from damp-chamber material (cashew 
nut), xX 500. 
Microconidia were plentiful in, as a rule, seven or eight days. 
Meanwhile, other tufts on the hypocotyl and roots grew rapidly, 
ore microconidia, and coalesced to form a whitish aerial 
mycelium covering the whole hypocotyl and roots in about 
eighteen days. Before this stage, macroconidia, measuring up 
to 27 by 2-5 and mostly three-septate, had been easily found. 
Tissue-fragments removed under sterile conditions from 
hypocotyls and roots were placed in prune decoction in petri 
