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quantity in eighteen days. In several cases, chlamydospore- 
formation took place in six or seven days. It was thus easy to 
obtain inoculum for pure cultures. 
The first pure cultures were made on banana plugs in sterile 
Roux tubes; the inoculum consisted of microconidia from the 
tissue-fragment cultures mentioned above. No mycelial growth 
was visible under four days, and it was then very sparse and 
white in colour. Microconidia were produced in eight days 
chlamydospores were seen. On slants of prune agar of 25° 
acidity in Fuller’s scale, white or pink-white aerial mycelium 
covered the whole surfaces of the media in three days and 
microconidia were then very plentiful. They were to be found 
particularly in moist oily superficial streaks resembling the 
growth of bacteria. Macroconidia (Fig. 2) were found a little 
later, and chlamydospores were numerous in twelve days. 
Fig. 2. Macroconidia from prune agar culture (cashew nut), x 500. 
After two months and more, the surfaces of the slants were 
masses of macroconidia in an apparently moist condition an 
of a yellow tinge, and after ten months they still kept that 
condition. The conidia were then capable of germinating 
rapidly overnight in a hanging-drop of distilled water. In prune 
agar plates, the growth and development were similar except 
that the mycelium became dark-grey in colour in parts. 
On glucose meat-extract agar of 30° acidity, growth was 
visible in twenty-four hours, and microconidia in forty-eight. 
The aerial mycelium was white and small in amount. After 
six days, the conidia were septate, and measured up to 38y long; 
in twenty days they were very numerous and four or five-septate. 
Chlamydospores were formed in twenty days, and, after that, 
the mycelium became dark in colour and apparently brittle, 
as if it were breaking up into resting portions. 
In French-bean agar of 20° acidity, the aerial mycelial growth 
was similar to that above, but larger and inclined towards a 
