273 
star-shaped flocculent habit such as was found on whole plants 
in the damp. Three-septate conidia were found after six days. 
‘They measured up to 35 in. length, and were in proportion 
broader than those of the glucose meat-extract culture above 
or of the rice agar culture below. Chlamydospores were formed 
in thirteen days, and a few days later were darker in colour 
than at first, of an average diameter of 10 (Fig. 3), and evidentl 
mature. 
Fig. 3. Mature chlamydospore from French-bean agar culture 
@ (cashew nut), x 500, 
In rice agar of 15° acidity, the aerial white mycelium bore 
microconidia in two days. Moist streaks were then quite 
apparent. In three days three-septate conidia were common, 
and in six days chlamydospores were very numerous. Later, 
the terminal chlamydospores were found to be in many cases 
two-celled (Fig. 4), and even three-celled (Fig. 5), in which case 
the aggregation resembled a spore of Piricularia oryzae, Br. and 
Cav., the cause of the blast disease of rice. 
Figs. 4-5. Chlamydospore-formation in a rice agar culture (cashew 
nut), x 500. 
These chlamydospores were found while conidial production 
was still in progress. Meanwhile, the aerial growth of mycelium 
arranged itself along definite concentric rings, and the conidia 
became so numerous that they covered the surface of the medium 
as in the prune agar slants mentioned. The largest measured 
32u long. The chlamydospores when placed in hanging drops 
germinated in forty-eight hours to give mycelium which produced 
further chlamydospores, terminal and intercalar and small, for 
they measured only up to 5y in diameter, or directly to produce 
microconidia (Fig. 6), the latter condition being the more common. 
The contents of the chlamydospores were coloured brown when 
treated with two per cent. osmic acid, while mycelium and 
conidia were unaffected. The mycelium in all the above cultures 
was typically that shown by van der Bijl in the plates attached 
to hi * 
ree aie months and longer, the above cultures which had 
been kept in the meantime at laboratory temperature (an average 
* loc. cit., plates 37-40. 
z 18834 - 
