274 
of about 25° Centigrade), as indeed all the cultures were kept, 
were found to be in a moist condition. No perithecial formation 
was noted, but minute brownish aggregations of hyphae occurred _ 
over the surfaces of the media, more particularly in the case of 
the glucose meat-extract agar.. In all the agar cultures, there 
were also the distinct surface lines of growth which resembled in. 
a striking manner the growth of bacteria. These lines which 
Chlamydospores, especially when formed intercalarly, were 
numerous in all the old cultures, and frequently occurred in 
groups of three or more; their walls averaged 1-5y in thickness 
o separation had taken place between the component parts 
of the two-celled terminal chlamydospores and their germination 
was observed to result in only one filament (Fig. 7). 
. 
Figs. 6-7. Germination of chlamydospores, 48 hours (cashew nut), 
x 500. 
_ The Fusarium was also grown with success on the sterilised 
surfaces of cashew nut seedling hypocotyls split longitudinally 
and carefully steamed. These cultures were kept in moist petri? 
dishes. The behaviour of the fungus was in all respects similar 
to that in the agar cultures except that the chlamydospore form : 
was not found. In one case, the mycelium assumed a pale blue 
colour with white edge, as if alkali had been added by accident. 
to part of the surface of the medium. The blue colouration | 
eventually gave place to honey-yellow, the prevailing colour in 
these cultures, although the white-pink colouration was by no. 
means wanting. 
Attempts were made to grow the fungus in sterilised soil 
moistened with sterile distilled water. Small quantities of the 
soil convenient for examination were placed in petri dishes and 
inoculated with conidia and mycelium from the agar cultures. 
Similar cultures were instituted with unsterilised soil from a» 
locality presumably free from the Fusarium wilt. No success — 
was met with until the cultures were incubated at 30° C, when 
hyphae were found spreading in all directions from the 
points of inoculation. Characteristic mycelium and microconidia © 
of the Fusarium were also found on organic fragments in _ 
soil of the cultures. No other spore stage of the fungus was » 
seen. The unsterilised soil cultures were much more Sarat 
than the others. 
