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INFECTION EXPERIMENTS WITH CASHEW NUT PLANTS. 
The effects of the disease had been so disastrous that only 
eight plants were available for inoculation experiments. The 
experiments were therefore less numerous than were wished. 
[he plants were healthy seedlings about ten inches high with 
two pairs of leaves and all were in pots. The inoculum in all 
cases was drawn from the prune agar slant cultures for the reason 
that the conidia of those cultures germinated in water more 
vigorously than the conidia of the others. A suspension of spores 
in sterile distilled water was used, and the viability of the spores 
was tested in the same medium. After necessary wounds had 
been made and the inoculum placed in position, disturbed 
tissues were carefully replaced. All the plants were placed 
out-of-doors after a day in the laboratory. 
The inoculum was placed in contact with lateral and tap 
roots of one plant, in wounds on a tap root and lateral roots of 
another, in contact with a hypocotyl under the soil level and in 
a wound in a hypocotyl under the soil level. It was also placed 
in the soil one half inch from roots, and one half inch from a 
hypocotyl. Two plants were kept as controls, and they remained 
healthy throughout the experiments. 
- the cases of the plants the roots of which, both lateral and 
main, were treated, in one case unwounded and in the other 
‘stad leaf-wilting began in five days and after ten days both 
plants had collapsed. The hypocotyls of both were then soft and 
watery. After three more days, both plants were dug up, care- 
fully sterilised, and placed in moist chambers. It was noted that, 
in the ease of the unwounded plant, the smaller lateral roots had 
decayed while the tap-root was still healthy and firm, and that, 
in the case of the wounded tap-root, the tissues had collapsed. 
On the surface of the soil of the pot of the unwounded plant, 
Fusarium mycelium and typical microconidia up to 10u long were 
found. After only twenty-four hours in the damp, both plants 
showed mycelium on the hypocotyls with numerous microconidia. — 
Three- to five-septate macroconidia up to 60 (fig. 9) and 
chlamydospores then appeared, and four days later seven-septate 
conidia (fig: 10) were in abundance. These large multi-septate 
Figs. 9-10. Large macroconidia from an inoculated plant (cashew 
nut), x 500. 
