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mycelium was pale grey-pink with white flakes. It eventually 
became dirty yellow. All three spore forms were produced. 
11. Acid potato (soaked for twenty minutes in 1 % sulphuric 
acid, rinsed, and boiled in distilled water).—In a few days white 
mycelium bearing microconidia was plentiful. This became 
faintly pink in colour and, later a pronounced salmon-pink. 
Coremial strands of mycelium were numerous and darker in 
colour than the mass. All three types of spores were produced. 
. Alkaline potato (soaked in 1 % sodium carbonate, rinsed, 
and boiled in distilled water)—The growth was not so dense as in 
Nos. 10 and 11, but micro- and macroconidia were found in plenty 
and a faint pink colour was developed. Chlamydospores were 
formed later. 
13. Normal rice paste (boiled in distilled water) —Growth 
was immediate and copious. Spores were very numerous; the 
macroconidia measured up to 30. long after three days. Later 
the prevailing colour was pale pink. The colour became deeper 
and towards the edges of the areas of growth shaded through 
salmon-pink into a pale orange. Strands of mycelium were 
common, as in No. 11. Spore formation continued to be pro- 
fuse, and chlamydospores were numerous after two months. 
14. Acid rice paste (a few drops of 5 % hydrochloric acid 
added to a small quantity of rice moistened with distilled water). 
—There was no difference between this culture and No. 13 save 
that the colour did not become so deeply red. 
15. Alkaline rice paste (a few drops of a saturated solution 
of sodium carbonate added to a small quantity of rice as in No. 14). 
—The growth was not so profuse as in Nos. 13 and 14, and the 
colour was slightly paler than that of No. 14.: 
16. Boiled tapioca paste—Growth was slow. Only one small, 
pinkish area of mycelium-bearing microconidia was noted. 
The fungus had already been grown on banana plugs, prune 
agar, glucose, meat-extract agar, French-bean agar, rice agar and 
sterilised hypocotyls of cashew nut seedlings, and its general 
characteristics in these cultures have already been reported. 
Small differences between the cultures of Butler and the special 
cultures existed, e.g., in the mycelial colours of Nos. 3, 4, and 5, 
in the formation of macroconidia in No. 5, in the lack of spores 
in No. 8, in the growth and formation of chlamydospores that 
took place in No. 9, in the occurrence of growth in concentric 
raised lines in Butler’s standard solution and asparagin culture 
whereas the same formation occurred only in the standard 
solution and saccharose culture, and in the absence of the pi 
colouration of the sclerotoid bodies which Butler noted in his 
acid potato and plantain cultures. With regard to sclerotoid 
bodies, similar stromata consisting of pseudo-parenchyma were 
formed in a prune agar tube culture after eight months, but 
they were of a dull grey-pink rather than a pure pink colour. 
They gave rise to conidiophores bearing microconidia, and 
chlamydospores were associated with them in great numbers. 
