231 
water, and roughly dried by pressing between cloths. After this 
no further exudation was produce 
s the experiments described above were not numerous, it is 
desirable that others should be carried out. These might include 
the treatment of twigs collected earlier in the year than Sep- 
tember. The addition of a poison to the solution of caleium 
chloride may also be recommended, as it was noticed that leaf- 
boring insects, which were present in some of the specimens; 
continued their burrows for a considerable time after the treat- 
ment with calcium chloride had been completed. 
The three following experiments in treating beech twigs with 
calcium chloride were also made: — re end of a twig was 
placed in a solution of calcium chloride in 50 per cent. spirit and 
left for some days, just as in the experiments with the watery 
solution; (2) a small specimen was kept immersed in a watery 
solution of calcium chloride for several days; (3) a specimen 
was boiled for a few minutes in the watery solution. These 
experiments did not give any satisfactory or even promising 
results, and were not repeated. 
n addition to beech, twigs of hornbeam, bay-laurel, and elm 
were placed with their cut ends in a solution of calcium chloride. 
Fairly good preservation was obtained in the case of hornbeam, 
though the leaves assumed a rather dingy appearance, but the 
method appears unsuitable for bay and elm. 
ew experiments were made in dyeing leaves of beech and 
hornbeam previously preserved by treatment with calcium 
chloride. The stain was applied by immersion in a watery 
solution of the dye, after which the leaves were rinsed in water. 
was found, however, that if the leaves were left for an hour 
or two in the stain they were liable to curl on drying. probably 
owing to loss of calcium chloride. Hence, if rapid staining 
cannot be carried out, experiments might be made on the 
feasibility of dyeing specimens first and then treating them with 
calcium chloride. 
XXV.—DIAGNOSES AFRICANAE: LXX. 
1611. Olax insculpta, Hutchinson ex E. G. Baker in Cat. 
Pl. Talb. Nigeria, 124, nomen [Wlacaceae-Olaceae]; affinis 
O. Mannii, Oliv., sed foliis latioribus plerumque ovato-orbicu- 
laribus nervis lateralibus profunde insculptis, floribus majoribus 
differt. 
eraciles 
Frutex glaber; ramuli flexuosi, parce foliati, g ; 
