130 
it would appear that only one aoe in each sterigma; each of the 
sporidia also contains a single nucleus, this resulting from the 
division of the nucleus in the eiatiraia the formation of the 
w settee a appears, therefore, to be identical with that of the 
nidia of Penicillium glaucum described by Dangeard and 
Gué en. The mature sporidia each contain a single nucleus, 
situated at the periphery, and a vacuole occupying the greater 
part of their volume; one or two metachromatic granules are also 
to be observed in the ‘peripheral layer of cytoplasm. 
““In this medium (distilled water) the osmotic pressure of 
which is very feeble, many curious morphological phenomena are 
Istvanffi*. Microsporogeny only occurred when the fungus was 
grown in glycerine, and the foll oan characteristic forms were 
xhibi poston resemblance to a promycelium 
(type Sclevotin ta); (b) 8 Patem on the sterigmata in the 
shape of a sickle (faucille) ; “cel arge masses of the type gamo- 
meterephal-eeneua Ns (d) little chains at the tips of fila- 
IL the piled be authors regarded this type of spore aad 
as a very rare and abnormal or bizarre occurrence, and in no case 
was the deve oye of in microconidia obse 
In 1908 Brookst published his ‘‘ Observations on the Biology 
of Botrytis aad aay and found that microconidial formation 
oceurred when the fun ngus was cultivated upon bouillon with 10 
per cent. gela i He states that in this medium ‘‘the growth 
was comparatively feeble, and during the first puree generations 
red conidiophores bearing microconidia were bor In the 
urth generation of the fungus upon the b bousllon medium the 
Browt was more fearant and the normal conidiophores were 
pats uced. It is not known whether this senge 1 in the mode of 
reproduction was dependent upon some alteration in the condi- 
tions of experiment, such as accounts for the different modes of 
reproduction in some algae, as Klebs has shown. If this were the 
ease the alteration must have been a slight one, for the cultures 
were kept in a room where the obvious physical ga controlling 
the growth were the same. It may be that the fungus, having 
become scene’ during three previous generati to the 
bouillon medium, was sufficiently invigorated to produce again 
the ietoia ao of conidiophore.’ 
* Istvanffi, Gy. de,: Anns. de !’Inst. Centr. Ampel. Roy. Hongr. iii. 1905. 
tt should be foe that raechictg the whole of his work ead ee 
‘Botrytis cinerea as identical with Sclerotinia Fuckelian. In view of the very 
ba discrete eae without genetic relationship, and belonging 7 
widely se separated grou See Li nd, J.—Danish Fungi in the Herbarium 
s nu p, Copen! agen: 1918 Pethy brides: G. H—Journ Dept. 
Treleed’ xi. No. 3, 1911; xvi. No. ‘4, 1916; Smith, R. E.—Bot. Cae. Xxix. 1900. 
+ Brooks, F. T.: Ann. Bot. xxii. 1908. 
