The adult organisation of Paragordius varius. 391 
iron-haematoxylin (Fig. 43, Pl. 39; Fig. 52, Pl. 40). They appear 
most frequently grouped in pairs. These are apparently not morpho- 
logically connected with the roots of the tubercles (Tub Figs. 43, 52) 
of the outer cuticle, though they stain in the same manner. Similar 
bodies are found in the tail lobes of the female, but less abundantly 
and there in some cases they appeared to be connected with the 
hairs of the outer cuticle. 
Outer cuticle of the female. This (Pl. 39, Figs. 37, 41) 
bounds the outer surface of the fibrous cuticula (F. Cut), and consists 
of an outer homogeneous layer (H. Cut) and an inner areolar layer 
(A. Cut). The former is a thin layer, dense and homogeneous, found 
at all portions of the external body surface; it differs chemically from 
the fibrous cuticula, and evidently is the first portion of the cuticula 
to be formed. At the points just above the lines of separation of the 
underlying areoles it shows thickenings (Fig. 37); on the outer surface 
of the tail lobes (Fig. 41) and on the mid-ventral region of the trunk 
these thickenings are more pronounced, appearing as little cones. On 
both the outer and the inner surfaces of the tail lobes delicate hairs 
project outward from this homogeneous layer (Fig. 41). By the use 
of the iron-haematoxylin stain it can also be determined that the 
inner surface of the homogeneous layer, particularly in the region of 
its thickenings shows on cross section rounded and rod-shaped, deep- 
staining bodies (Figs. 37, 41). These are arranged in a single layer, 
are mainly interareolar in disposition, and appear to be a sculptur- 
ation of the inner surface; where the areolar layer is lacking beneath 
the homogeneous layer of the cuticle, the layer of these small bodies 
is continuous. The areolar layer of the outer cuticle (Figs. 37, 41 
A. Cut) is lacking only on the anterior (Fig. 10, Pl. 37) and ventro- 
lateral regions of the head, on the mid-ventral region of the trunk, 
and on the inner (cloacal) surfaces of the tail lobes. In it are found 
large homogeneous bodies (Pl. 39, Figs. 37, 41 Ar) which correspond 
to the areoles of surface views; they are largest on the outer surfaces 
of the tail lobes (Fig. 41). On surface view they appear rounded or 
oval, and more or less quadrilateral on cross sections of the body. 
They show more affinity for haematoxylin than for eosin. The nature 
of these areoles is hardly to be determined from the adult anatomy ; 
but it may be suggested that they represent masses of glandular 
secretion poured out by the hypodermal gland cells (Fig. 41 Gl. C), 
after the homogeneous layer of the outer cuticle has been formed, 
and before the development of the fibrous cuticle. 
Zool. Jahrb, XVIII. Abth. f. Morph. 26 
