The finer Structure of the Nerve Cells of Invertebrates. 31 



under a low power, and shows, as in Fig. 1, a marked difference in 

 the manner in which its constituent parts are stained. It is seen that 

 the cell body appears to be stained chiefly by the sa- 

 franin, while the axis-cylinder process and neuroglia 

 tissue are stained by the light green; that the chroma- 

 tin granules in the nucleus are, in this particular in- 

 stance, stained by the safranin which is a nuclear stain; 

 and that the nucleoli and neuroglia-nuclei, whi ch often 

 resemble each other in form and size, are also stained 

 deeply by the safranin. 



The two larger cells shown in Fig. 5 are particularly worthy of 

 notice, as they represent two modifications of the manner in which 

 the chromophilous granules may be distributed in the cell body. In 

 the large cell, at the right side of the figure, which is a longitudinal 

 section to one side of the axis-cylinder process, the ectoplasmatic 

 layer is seen to be free from chromophilous granules, and to be stained 

 exclusively by the green both at the axis-cylinder pole of the cell, 

 and on the side of the cell directed towards the upper margin of the 

 plate. The other cell in which the axis - cylinder process is shown, 

 is of about the same type as that represented by Fig. 2. 



This safranin-light green combination has proved particularly 

 valuable in corroborating the results obtained by the methylen blue- 

 eosin method, on account of the analogous manner in which the cell 

 contents are differentiated from each other. 



Fig. 6, which is a camera drawing of a highly magnified ganglion 

 cell of Helix (sub,, safranin-light green), 3hows that the red a p - 

 pearance presented by the cell body in Fig. 5, is due 

 to the presence therein, of a large number of small 

 granules which are deeply stained by the safranin. It 

 is also to be observed that the substance of the cell 

 body betweenthegranulesis stained by the light green. 



Fig. 7 also represents a highly magnified ganglion cell of Helix 

 (Flemming's sol., saf.-light green), and shows a similar differentiation 

 of the cellular contents into a granular substance in the cell 

 body which stains red, and an intergranular substance 

 which stains green. 



Before passing to a further study of these granules , as seen in 



accounts for the promiscuous manner in which they have been used for 

 illustrating the same points. 



