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On the Artificial Culture of Marine Plankton 

 Organisms. 



By 

 E. J. Allen, D.Sc, 



Director of Laboratories and Secretary to the Council of the Marine Biological Association, 



and 



E. W. Nelson, 



Assistant Naturalist. 



Introduction .... 

 I. Cultuie of Plankton Diatoms 



A. Practical Culture Methods 



1. Miquel's Method 



2. Houghton Gill's Method 



3. [a) Modification of Miquel's Method. 

 [h) English Channel Water . 



(c) Tank- Water 



(d) Animal-Cliarcoal Water . 



(e) Peroxide of Hydrogen Water 

 (/) Cultures in these Media . 



B. Experiments with a view to Determining 



the successful Culture of Diatoms 



Methods 



The Sea-water employed 



The Constituents of Miquel's Solutions 



Animal Charcoal and Peroxide of Hydroge 



Reviving Exhausted Cultures 



Silica 



Organic Infusions 



Artificial Sea-water 



Alkalinity . 



Salinity 



Light 



Temperature 



General Conclusions 

 II. Mixed Cultures 

 III. Notes on particular species of Diatoms, on their 



on other Algte occurring in Cultures 

 IV. Rearing of Marine Larv;i3 

 Methods 

 Echinus acutus . 

 E. esculentus 

 E. miliaris 

 Cucumaria saxicola 

 Pomatoceros triqueter 

 C'haetopterus variopedatns . 

 Sabellaria alveolata 

 Archidoris tuberculuta 



' Miquel Sea-water 



the Conditions which underlie 



methods of Reproduction, and 



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