58 ANIMAL COLOURING MATTERS EXAMINED AT THE 



I find actinioliasmatin present, differing in no essential respect 

 from that colouring matter as I have described it elsewhere.* 



In Tiihularia indivisa the bright red colour of the polyp heads 

 was found to be due to a lipochrome. 



Vermes. — In Arenicola, as in Lumhricus terrestris, the glandular 

 tissue surrounding the intestine was found to contain a lipochrome, 

 as well as the integument. From the latter situation the black 

 pigment could be extracted by caustic potash, but in solution 

 it gave no bands. Haemoglobin is present in this worm, as is 

 known by Prof. Ray Lankester's researches. 



In Terehella, besides the haemoglobin (Lankester), to which, 

 and not to tetronerythrin the colour of the tentacles is due, the 

 integument contains a lipochrome. In Cirratulus the tentacles also 

 owe their colour to oxyhaemoglobin, but they also yield a lipochrome 

 to solvents, as does the integument. 



In Nereis a lipochrome is also present besides the hsemoglobin. 

 The blood of Nereis Dnmerilii showed one broad band like that of 

 reduced haemoglobin. In some parts of the worm only one band 

 like the first one of oxyhgemoglobin was seen. An aqueous solution 

 of the blood, however, gave two bands, but on adding ammonium 

 sulphide they disappeared, and did not seem to be replaced by the 

 single band of reduced heemoglobin. 



In Pontobdella an undescribed colouring matter bearing a remote 

 resemblance to chlorophyll can be extracted from the integument 

 by absolute alcohol. This comes from the large green -coloured 

 corpuscles situated in the deeper parts of the integument.f The 

 green solution showed no red fluorescence, nor did it show all the 

 chlorophyll bands. With hydrochloric acid it became a deeper 

 blue colour, but did not show the phyllocyanin spectrum. 



In Polynoe a phosphorescent area was noticed surrounding the 

 head ganglion, which latter was of a red colour, and gave a band 

 situated approximately in the same part of the spectrum as that of 

 reduced haemoglobin, but in no part of the worm could I see the 

 spectrum of oxyheemoglobin. In one specimen the cerebral ganglion 

 showed one band like the first of oxyhaemoglobin, and in another 

 this ganglion was yellow and showed no baud. 



I have examined the green colouring matter of Ohsetopterus 

 insignis (Baird) and can confirm Professor Lankester's statement that 

 it is chlorophyll. The alcohol solution possesses a fine red fluores- 

 cence and gives all the chlorophyll band and yields " modified " and 

 '' acid " chlorophyll as well as phyllocyanin by suitable treatment. 



* Philos, Trans., Pt. ii, 1885. 



t See A. GJbbs Bourne's paper, Quart. Journ. Mic. Sci., July, 1884. 



