PLYMOUTH MARINE BIOLOGICAL LABOEATOEY. 69 



I could detect no haemoglobin in Nemertes Neesii, nor did the integu- 

 ment yield hgematoporphyrin to acidulated alcohol. 



In Phyllodoce virtdis a special green pigment occurs which is not 

 chlorophyll. This pigment is described at length in the complete 

 paper. It may be remembered that P. Geddes* exposed this green 

 polychsete Annelid to sunlight and failed to get any evolution of 

 oxygen. Of course the above result explains why. 



Tunicates : In Styela grossularia the brilliant red pigment sur- 

 rounding the exhalent and inhalent orifices was found to be a red 

 lipochrome, which by Merejkowskif would doubtless be taken for 

 tetronerythriuj but in this, as well as in many other instances, it is 

 more closely related to Kiihne^s rhodophan than to anything else. 

 The ether solution was at first a fine red colour, but soon changed 

 to greenish, and although it did not show a red fluorescence it gave a 

 chlorophylloid spectrum. But the pigment present was not chloro- 

 phyll J it seemed to possess characters, however, which should class 

 it among the lipochromes and which show that the step from one to 

 the other is not a great one. 



Botryllus violaceus yields to solvents such as alcohol and ether a 

 yellow lipochrome, which in a deep layer of alcohol solution showed 

 a band in red like that of chlorophyll. It is probable that in this 

 instance also a colouring matter is present possessing some of the 

 characters of chlorophyll and being yet a lipochrome. J 



In Botrylloides an allied pigment is present. 



In Clavellina lepadiformis some bluish colouring matter occurs 

 which showed some shading at the blue end of green and a feeble 

 shading at D, but I was unable to examine it further. 



In Ascidia virginea a reddish pigment was noticed which gave two 

 shadings in the green and strongly absorbed the violet end of the 

 spectrum, but I failed to get it into solution. It is probably, how- 

 ever, related to the lipochromes. 



Bryozoans : The examination of Lepralia foliacea yielded interest- 

 ing results. It contains abundance of chlorophyll mixed with a 

 lipochrome, to which latter the fine orange of this species is due. 

 The chlorophyll is also accompanied by a second pigment, probably 

 chlorofucin,§ if so, the latter must be due either to symbiotic algae 

 or to brown marine algae. The ether solution shows the bands of 

 the latter pigment well marked, and has a red fluorescence, which is 



* Proc. Roy. Soc. Edin., vol. xi, 1881-82. 



t Bullet, de la Societe zoolog. de France, 1883, p. 81 et seq. ; cf. Dr. W. Warm in 

 Jahreshefte des Vereins fiir vaterl. Naturkunde in Wiirtt., 1885, S. 262 — 265. 



1 Cf. Krukenberg, Verg. physiol. Stud., 2 Reilie, 3 Abth., 1882, Tafel v, 7. 



§ Sorby, Proc. Roy. Soc, No. 146, vol. xxi, 1873 j see also my paper iu Quart. Journ. 

 Mic. Soc, vol. xxvii. 



