168 NOTES AND MEMORANDA. 
Three of the males were ripe, and the other three probably 
became ripe later in the season. Two of the females were nearly 
ripe, and the smallest was killed and found to be still immature. 
The flounders in the large tank mentioned in the previous paper 
were examined on May 13th. There were fifty altogether surviving 
out of sixty-five counted in the spring of 1892. Thirty-two of the 
fish were measured and examined ; the sizes ranged from 5 inches to 
113 inches. As it was late in the season many of these fish may 
have finished spawning, only two females showed reproductive 
activity, one was ripe, and another nearly so. Owing to pressure 
of other work it was not possible to give more attention to these 
experiments, but I have thought it worth while to record the sizes 
and the indications as to the relation between age and breeding.— 
J.T. C. 
Rearing of Fish-larve.—F rom the ripe flounders among the number 
reared in the aquarium and then three years old last spring I took 
a number of healthy eggs, and fertilised them. ‘The first lot were 
hatched on April 20th. They were kept in one of the boxes 
belonging to the Dannevig apparatus, placed in one of the laboratory 
tanks. On the 22nd I turned the larve out of the box into the tank, 
protecting the overflow pipe by means of a bolting cloth screen, and 
keeping a slight but constant inflow of water into the tank. On the 
24th the yolk was almost entirely absorbed, and I put in as food 
some of the minute suspended particles obtained by stirring up finely 
minced worm in a jar of sea-water. The little fish took this food 
readily, and could be seen deliberately pecking at the particles in 
the water. They lived and seemed healthy until April 28th, but then 
began to diminish in numbers, and on May Ist few were to be seen. 
Another lot of eggs procured from the same source were hatched 
on April 29th, and turned into a tank arranged in the same way two 
days afterwards. They began to feed on May 4th, and lived well 
until May 9th, when the numbers began to diminish. I found the 
dead ones sticking to the screen which protected the outflow. On 
May 15th two were seen still alive, fourteen days old, and after this 
date none were left.—J. T. C. 
