460 W. DE MORGAN AND THE LATE G. HAROLD DREW. 



Experiment 13. — 17th January, 1912, 4. p.m. Flourishing colony of 

 Antennularia ramosa squeezed through 50 mesh and 180 mesh into watch- 

 glasses. Allowed to settle for 3 hours, and then transferred to bowls of 

 Berkefeld water. 



18th, 10 a.m. All the 50-mesh cultures show typical lobate masses, 

 but of smaller size than in Exp. 10. The masses from 180 mesh are 

 smaller and lighter coloured. Pieces of tentacle and theca were observed, 

 and removed. Small masses from each culture placed in finger-bowls 

 containing about 450 c.c. Berkefeld water. 



19th. All masses from 50 mesh have contracted greatly, and in- 

 creased in length vertically. They are generally conical. In places 

 the perisarc not formed. Colour, a healthy yellow. All adhered to 

 the glass. 



The culture from 180 mesh differs from above. Only small spherical 

 masses have resulted, generally adhering to the glass. Perisarc has not 

 yet been formed. 



20th. Most of the masses have contracted further, but the majority 

 are grey and unhealthy looking. 



It may be noted that the Laboratory was particularly warm at this 

 time, and the cultures were affected thereby. 



24th. Most of the masses were dead, one or two remained alive until 

 31st. They showed no great change except contraction of the interior 

 protoplasm away from the perisarc and slight attempts to form knobs 

 or proliferations. These did not advance far, and the plasm soon con- 

 tracted away from the surrounding perisarc. Certain of the masses were 

 fixed for examination. 



Experiment 15. — 25th January, 1912. Antennularia ramosa squeezed 

 through 50 mesh and Antennularia antennina through 180 mesh. Allowed 

 to settle through the day. 



26th. The 180-mesh culture has simply formed a film over the bottom 

 of the glass — no masses have formed. 



Numerous yellowish masses in the 50-mesh culture. Certain of these 

 were transferred to finger-bowls of " outside " water. Generally they 

 were not healthy in appearance, and a good deal of foreign matter was 

 mixed with them. 



28th. One mass remains healthy. Several lobes appear on it. The 

 perisarc has formed, but is rougher than usual. A great many Infusoria 

 in the cultures. 



29th. The lobes have contracted into the main mass, which has also 

 further contracted. 



