282 CHARLES HOWARD EDMONDSON 



In each individual the organ lay on the right typhlosole, occiipying- 

 nearly the entire length of the tube, but did not protrude into the 

 stomach (fig. 24). In diameter the style measured 0.5 mm, at the dis- 

 tal extremity, which at this time was somewhat thicker than the proxi- 

 mal end. That no food had been taken by the animal during the in- 

 terval of thirteen days seemed apparent by the empty and blanched 

 condition of the intestines. The stomach was Avithout food in each 

 surviving individual. 



The material forming the food core of the crj^stalline style at this 

 early period of its development is probably furnished from that which 

 was in the stomach at the time of the extraction of the style. Although 

 the style sac is an open tube leading from the stomach after the re- 

 moval of the crystalline stjde, in no specimen examined have I been 

 able to detect a particle of food or foreign material being carried down 

 this tube from the stomach except that enclosed by the style itself. 



Experiment 3. February 23, 1918 



Twelve clams, ranging in size from 40 to 125 mm., with styles re- 

 moved, as in the preceding experiment, were planted under normal 

 conditions. This test extended for a period of sixteen days. 



Result, March 9, 1918. On this date nine of the twelve clams were 

 surviving and apparently in good condition. The cut surfaces were 

 well healed, and in a number of specimens the style sacs were also 

 closed at the severed ends. On opening the proximal portion of the 

 style sac of each of these clams no appearance of a regenerating crystal- 

 line style was evident. The tube was free of foreign material, but ap- 

 parently the reformation of the crystalline style had not yet made its 

 beginning. An examination of the stomach revealed the absence of 

 food, and the intestinal tract was also empty. It was quite evident 

 that the clams had not been feeding since the removal of the crystalline 

 styles. This experiment resulted negatively in so far as the reforma- 

 tion of the crystalline style is concerned, but, I believe, is evidence of 

 the slow development of this organ during the winter months. 



Experiment 4- November 30, 1918 



Twelve clams with crystalline styles removed were taken from their 

 native bed and planted in a sandy locality near the bank of the river 

 where they would be exposed for a much longer period than usual by 

 the recession of the tides. The experiment continued for twenty-six 

 days. 



Result, December 26, 1918. Of the twelve clams planted in the sandy 

 soil near the shore line four were alive after a period of twenty-six 

 days. Of these survivors two specimens showed no evidences of the 

 reformation of crystalline styles. The other two possessed styles very 

 rudimentary in development, correspondng closely to those of six days'' 

 growth as recorded in experiment 1 . 



