STUDIES WITH SUDAN III IN METABOLISM AND 



INHERITANCE 



OSCAR RIDDLE 



Front the Laboratories of Experimental Therapeutics and Zoology, University of Chicago 



I. Introduction -. i6-! 



II. Historical 1 6 c 



1 Membranes through which Sudan is known to pass 165 



2 The mechanism of the transfer and deposition of Sudan within the body 166 



3 The uses to which Sudan has been apphed in experimental biology and medicine.. . . 167 



4 Sudan III and other pigments in "inheritance" 168 



III. Experimental methods 1 69 



IV. Results of experiments 170 



Deposition of Sudan III in ova lyo 



Deposition of Sudan III in the soma 171 



The rate and conditions of absorption and deposition of Sudan III 172 



V. An unsuspected action of Sudan. (An apparent tendency of Sudan to lessen the "avail- 

 ability" of fats in the organism) 17^ 



Biological evidence .... 1 74 



Chemical evidence 176 



VI. Discussion and critique 177 



VII. Summary 182 



VIII. Bibliography i8-i 



INTRODUCTION 



In a paper presented before the American Society of Zoologists, 

 December, 1907, the writer (07) described and demonstrated, 

 among other things, the deposit of Sudan III in the egg of the 

 domestic fowl. A very brief abstract of this paper was published 

 in Science, June, 1908. All of the data concerning the method of 

 introducing the color into the eggs, and the significanceof this in 

 metabolism and inheritance, as well as the data concerning an 

 interpretation of the white and yellow yolk of vertebrate ova, were 

 then written up together. It now seems advisable however, to 

 treat the first two subjects apart from the last since the phenomena 



